NBDC Research ID: hum0247.v1
SUMMARY
Aims: Establishment of a tumor infiltrating lymphocytes (TIL) therapy for malignant melanoma
Methods: Exome sequencing of melanoma tissues for TIL production and paired peripheral blood mononuclear cells (PBMC)
Participants/Materials: human melanoma and paired PBMC
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000249 | NGS (Exome) | Controlled-access (Type I) | 2021/01/04 |
| DRA010773 | NGS (Exome) | Unrestricted-access | 2021/01/04 |
*Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more
*When the research results including the data which were downloaded from NHA/DRA, are published or presented somewhere, the data user must refer the papers which are related to the data, or include in the acknowledgment. Learn more
MOLECULAR DATA
| Participants/Materials |
melanoma (ICD10: C43.3, C43.7): 3 cases tumor tissue: 3 samples non-tumor tissue (PBMC): 3 samples |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 1500] |
| Library Source | DNAs extracted from tumor tissues and PBMCs of melanoma patients |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon Kit V6 |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID / DDBJ Sequence Read Archive ID |
non-tumor tissues: JGAD000349 tumor tissues: DRA010773 |
| Total Data Volume |
non-tumor tissues: 25 GB (fastq) tumor tissues: 15 GB (bam [ref: hg19]) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Takashi Iwata
Affiliation: Department of Obsterics and Gynecology, Keio University School of Medicine
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED) | Pilot study of adoptive cell therapy using cultured tumor infiltrating lymphocytes with lympho-depleting non-myeloablative preconditioning for patients with metastatic melanoma | JP17ck0106158 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Pilot study of adoptive cell therapy using cultured tumor infiltrating lymphocytes for Japanese melanoma patients refractory to immune-checkpoint inhibitors | doi: 10.1111/cas.15009 |
JGAD000349 DRA010773 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
| Takuya Yamamoto | Laboratory of Precision Immunology, National Institutes of Biomedical Innovation, Health and Nutrition | Japan | Basic research for vaccine development against gastrointestinal cancer and malignant melanoma based on the identification of novel cancer antigens | JGAD000349 | 2024/11/12-2025/09/01 |
