NBDC Research ID: hum0223.v1

SUMMARY

Aims: To clarify of the disease status of myeloma and lymphoma by somatic gene mutation analyses

Methods: gene mutaion analyses using NGS technology at the point of diagnosis, relapse, or proceeding disease

Participants/Materials: multiple myeloma

Dataset ID	Type of Data	Criteria	Release Date
JGAS000232	NGS (Target Capture)	Controlled-access (Type I)	2020/09/01

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MOLECULAR DATA


Participants/Materials	multiple myeloma (ICD10: C90.0): 7 cases myeloma cells from bone marrow aspirates: total 30 samples
Targets	Target Capture
Target Loci for Capture Methods	126 genes
Platform	Thermo Fisher Scientific [Ion Proton]
Library Source	DNAs extracted from myeloma cells isolated from bone marrow samples at the point of diagnosis, relapse, or proceeding disease
Cell Lines	-
Library Construction (kit name)	Ion Ampliseq Designer™
Fragmentation Methods	-
Spot Type	Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	3-1754 bp
QC/Filtering Methods	TaqMan® Assay
Deduplication	-
Mapping Methods	TMAP
Reference Genome Sequence	hg19
Coverage (Depth)	2815
Detecting Methods for Variation)	Variant Caller
Japanese Genotype-phenotype Archive Dataset ID	JGAD000326
Total Data Volume	87 GB（vcf）
Comments (Policies)	NBDC policy

Principal Investigator: Yasuhito Terui

Affiliation: Dept of Hematology Oncology, The Cancer Institute Hospital of JFCR

Project / Group Name: -

Funds / Grants (Research Project Number):

Name	Title	Project Number
-

	Title	DOI	Dataset ID
1	The clonal evolution during long-term clinical course of multiple myeloma	doi: 10.1007/s12185-020-02979-7	JGAD000326
2

Principal Investigator	Affiliation	Country/Region	Research Title	Data in Use (Dataset ID)	Period of Data Use