NBDC Research ID: hum0203.v1



Aims: To clarify the genomic feature of Japanese multiple myeloma (MM) patients by comprehensive genetic analyses

Methods: Targeted capture sequencing

Participants/Materials: Japanese MM


Dataset IDType of DataCriteriaRelease Date
JGAS000206 NGS (Target Capture) Controlled-access (Type I) 2020/06/01

*Release Note

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MM (ICD10: C900): 154 cases

    primary tumor tissues: 154 samples

    recurrent tumor tissues: 6 samples

    peripheral blood mononuclear cells as a normal sample: 23 samples

Targets Target Capture
Target Loci for Capture Methods 340 genes + immunoglobulin variable regions (IGH, IGK, IGL)
Platform Illumina [HiSeq 2500]
Library Source DNAs extracted from frozen bone marrow CD138 positive cells isolated from bone marrow aspirates and peripheral blood mononuclear cells
Cell Lines -
Library Construction (kit name) SureSelect custom kit
Fragmentation Methods Ultrasonic fragmentation (Covaris ME220)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 100-200 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000291
Total Data Volume

255 GB

    Data files: 366 (fastq)

    Analysis files: 1 (vcf [ref: hg19])

Comments (Policies) NBDC policy



Principal Investigator: Keizo Horibe

Affiliation: Clinical Researc Center, Nagoya Medical Center

Project / Group Name: Program for an Integrated Database of Clinical and Genomic Information

URL: https://www.amed.go.jp/en/program/list/14/01/006.html

Funds / Grants (Research Project Number):

NameTitleProject Number
Program for an Integrated Database of Clinical and Genomic Information, Japan Agency for Medical Research and Development (AMED) Establishment of an integrated database for clinical and genomic data in cancer JP16kk0205005



TitleDOIDataset ID
1 Genomic analysis of multiple myeloma using targeted capture sequencing in the Japanese cohort doi: 10.1111/bjh.16720 JGAD000291


USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use