NBDC Research ID: hum0203.v1
SUMMARY
Aims: To clarify the genomic feature of Japanese multiple myeloma (MM) patients by comprehensive genetic analyses
Methods: Targeted capture sequencing
Participants/Materials: Japanese MM
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000206 | NGS (Target Capture) | Controlled-access (Type I) | 2020/06/01 |
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MOLECULAR DATA
| Participants/Materials |
MM (ICD10: C900): 154 cases primary tumor tissues: 154 samples recurrent tumor tissues: 6 samples peripheral blood mononuclear cells as a normal sample: 23 samples |
| Targets | Target Capture |
| Target Loci for Capture Methods | 340 genes + immunoglobulin variable regions (IGH, IGK, IGL) |
| Platform | Illumina [HiSeq 2500] |
| Library Source | DNAs extracted from frozen bone marrow CD138 positive cells isolated from bone marrow aspirates and peripheral blood mononuclear cells |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect custom kit |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris ME220) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100-200 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000291 |
| Total Data Volume |
255 GB Data files: 366 (fastq) Analysis files: 1 (vcf [ref: hg19]) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Keizo Horibe
Affiliation: Clinical Researc Center, Nagoya Medical Center
Project / Group Name: Program for an Integrated Database of Clinical and Genomic Information
URL： https://www.amed.go.jp/en/program/list/14/01/006.html
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Program for an Integrated Database of Clinical and Genomic Information, Japan Agency for Medical Research and Development (AMED) | Establishment of an integrated database for clinical and genomic data in cancer | JP16kk0205005 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Genomic analysis of multiple myeloma using targeted capture sequencing in the Japanese cohort | doi: 10.1111/bjh.16720 | JGAD000291 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
