NBDC Research ID: hum0201.v8

 

SUMMARY

Aims: Search for differences in genomics and traits among normal cells, digestive non-tumor cells (inflammatory cells), and tumor cells

Methods:

JGAS000199: The organoids were established from epithelial tissues from patients, and cultured. DNAs were extracted from the organoids and peripheral blood cells. Whole exome sequencing analysis was performed to identify the somatic mutations.

JGAS000237: Organoids were established from tissue samples (normal and cancer) obtained from any cancer patients. The organoids were cultured, and DNA / RNA was extracted from the organoids for whole genome / RNA sequencing analysis to identify the somatic mutations. Blood samples were substituted when normal tissue samples were not available.

JGAS000256: RNA was extracted from the 2D-cultured normal duodenal organoids with or without medium rotation. RNA sequencing was performed to identify the changes in gene expression after culture medium rotation.

JGAS000378: RNA sequencing and Chip-seq analysis for the organoids derived from colon cancer tissues and a normal epithelial tissue from patients

JGAS000350: RNA sequencing analysis for the organoids derived from colon cancer tissues

JGAS000550: single cell RNA sequencing analysis for normal colonic tissue and RNA sequencing or Whole Exome sequencing analyses for the organoids derived from healthy normal colonic tissues in the neoplastic disease patient

JGAS000719: Organoids were derived from surgically resected specimens, endoscopic ultrasound-guided fine needle aspiration samples, brushing samples, pleural effusion, and ascites of patients with pancreatic cancer. Genetically engineered human pancreatic duct organoids were made by introducing cancer gene mutations in duct organoids using CRISPR-Cas9. DNAs/RNAs were extracted from the organoids for whole exome sequencing, RNA sequencing, single cell RNA sequencing, ATAC sequencing, and ChIP sequencing analyses.

Participants/Materials:

JGAS000199: Controls who underwent colonoscopy, gastrointestinal inflammatory disease patients, and neoplastic disease patients

JGAS000237: Neoplastic disease patients

JGAS000256: Normal duodenum tissue from a neoplastic disease patient

JGAS000378: Colon cancer tissues and a normal epithelial tissue from neoplastic disease patients

JGAS000350: Neoplastic disease patient

JGAS000550: Neoplastic disease patient

JGAS000719: Pancreatic cancer patients

 

Dataset IDType of DataCriteriaRelease Date
JGAS000199 NGS (Exome) Controlled-access (Type I) 2019/12/20
JGAS000237 NGS (WGS, RNA-seq) Controlled-access (Type I) 2020/10/06
JGAS000256 NGS (RNA-seq) Controlled-access (Type I) 2020/11/20
JGAS000378 NGS (RNA-seq, ChIP-seq) Controlled-access (Type I) 2021/11/19
JGAS000350 NGS (RNA-seq) Controlled-access (Type I) 2022/06/06
JGAS000550 NGS (scRNA-seq, RNA-seq, Exome) Controlled-access (Type I) 2022/08/05
JGAD000687 Processed data of JGAD000335 by JGA Controlled-access (Type I) 2022/12/27
JGAS000719 NGS(Exome, RNA-seq, scRNA-seq, ATAC-seq, ChIP-seq Controlled-access (Type I) 2024/08/28

*Release Note

*Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more

 

MOLECULAR DATA

Exome (JGAS000199/JGAS000550/JGAS000719)

Participants/Materials

gastrointestinal inflammatory disease (ICD10: K51): 29 cases

gastrointestinal inflammatory disease with neoplastic disease (ICD10: K51, C18, C19, C20): 26 cases

organoids derived from healthy normal colonic tissues with neoplastic disease (ICD10: C18): 2 cases

controls who underwent colonoscopy: 16 individuals

pancreatic cancer (ICD10: C25): 50 cases

 (organoids derived from tumor tissues)

Targets Exome
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2500/4000, NovaSeq6000]
Library Source DNAs extracted from peripheral blood cells and organoids established from normal or inflammatory/tumor tissues of the patients or controls
Cell Lines -
Library Construction (kit name) SureSelect Human All Exon kit
Fragmentation Methods Ultrasonic fragmentation
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 200 bp
Japanese Genotype-phenotype Archive Dataset ID

JGAD000284

JGAD000669

JGAD000852

Total Data Volume

JGAD000284: 488 GB (fastq)

JGAD000669: 59.5 GB (fastq)

JGAD000852:846.3 GB(fastq)

Comments (Policies) NBDC policy

 

WGS (JGAS000237)

Participants/Materials

Neoplastic disease: 23 cases (55 samples)

    Small cell lung cancer (ICD10: C34)

    Biliary tract cancer (ICD10: C23, C24, C78)

    Colon cancer (ICD10: C18-20)

    Duodenal cancer (ICD10: C17)

    Esophageal cancer (ICD10: C15)

    Stomach cancer (ICD10: C16, C78)

    Liver cancer (ICD10: C22)

    Pancreatic cancer (ICD10: C25)

Targets WGS
Target Loci for Capture Methods -
Platform Illumina [HiSeq X Ten]
Library Source DNAs extracted from peripheral blood cells and organoids established from normal and cancer tissues of the patients
Cell Lines -
Library Construction (kit name) Library was constructed by BGI
Fragmentation Methods Ultrasonic fragmentation
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 300 bp
Japanese Genotype-phenotype Archive Dataset ID

JGAD000335

Dataset ID of the Processed data by JGA

JGAD000687

The way to Process

Total Data Volume 2.752 TB (fastq)
Comments (Policies) NBDC policy

 

RNA-seq (JGAS000237, JGAS000378)

Participants/Materials

Neoplastic disease: 23 + 4 cases (35+ 5 samples)

    Small cell lung cancer (ICD10: C34)

    Biliary tract cancer (ICD10: C23, C24, C78)

    Colon cancer (ICD10: C18-20)

    Duodenal cancer (ICD10: C17)

    Esophageal cancer (ICD10: C15)

    Stomach cancer (ICD10: C16, C78)

    Liver cancer (ICD10: C22)

    Pancreatic cancer (ICD10: C25)

Targets RNA-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2500/X Ten, NovaSeq 6000]
Library Source

RNAs extracted from organoids established from tumor tissues and a normal epithelial tissue of the patients

RNAs extracted from organoids with BET bromodomain inhibitor established from tumor tissues and a normal epithelial tissue of colon cancer patients

Cell Lines -
Library Construction (kit name)

TruSeq RNA Library Prep Kit v2

TruSeq Stranded mRNA Library Prep Kit

Fragmentation Methods Heat treatment
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 250 bp/150 bp
Japanese Genotype-phenotype Archive Dataset ID

JGAD000336

JGAD000492

Total Data Volume

JGAD000336: 2.752 TB (fastq)

JGAD000492: 190.5 GB (fastq)

Comments (Policies) NBDC policy

 

RNA-seq (JGAS000256)

Participants/Materials Neoplastic disease (ICD10: C16): 1 case (1 sample)
Targets RNA-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq X Ten]
Library Source Total RNA extracted from normal duodenum organoids with or without a four-day medium rotation, and after 0, 1, 2 or 4 days of medium rotation
Cell Lines -
Library Construction (kit name)

TruSeq RNA Library Prep Kit v2

TruSeq Stranded mRNA Library Prep Kit

Fragmentation Methods Heat treatment
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 250 bp/150 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000359
Total Data Volume 37 GB (fastq)
Comments (Policies) NBDC policy

 

RNA-seq (JGAS000350)

Participants/Materials Neoplastic disease (ICD10: C16): 2 case (11 sample)
Targets RNA-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 4000]
Library Source RNAs extracted from organoids derived from colon cancer tissues
Cell Lines -
Library Construction (kit name)

TruSeq RNA Library Prep Kit v2

TruSeq Stranded mRNA Library Prep Kit

Fragmentation Methods Heat treatment
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 250 bp/150 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000464
Total Data Volume 108.9 GB (fastq)
Comments (Policies) NBDC policy

 

RNA-seq (JGAS000550)

Participants/Materials Neoplastic disease (ICD10: C18): 2 case (8 sample)
Targets RNA-seq
Target Loci for Capture Methods -
Platform Illumina [NovaSeq 6000]
Library Source Total RNA extracted from normal duodenum organoids with or without a four-day medium rotation, and after 0, 1, 2 or 4 days of medium rotation
Cell Lines -
Library Construction (kit name)

TruSeq RNA Library Prep Kit v2

TruSeq Stranded mRNA Library Prep Kit

Fragmentation Methods Heat treatment
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150 x 2 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000669
Total Data Volume 46.6 GB (fastq)
Comments (Policies) NBDC policy

 

RNA-seq (JGAS000719)

Participants/Materials

Pancreatic cancer (ICD10: C25): 40 case (50 sample)

        organoids derived from tumor tissues: 38 cases (38 samples)

        genetically engineered pancreatic duct organoids: 2 cases (12 samples)

Targets RNA-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 4000, NovaSeq 6000]
Library Source RNAs extracted from pancreatic cancer organoids or genetically engineered pancreatic duct organoids
Cell Lines -
Library Construction (kit name)

TruSeq RNA Library Prep Kit v2

TruSeq Stranded mRNA Library Prep Kit

Fragmentation Methods Heat treatment
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150 x 2 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000852
Total Data Volume 846.3 GB (fastq)
Comments (Policies) NBDC policy

 

scRNA-seq (JGAS000550)

Participants/Materials Neoplastic disease (ICD10: C16): 1 case (1 sample)
Targets scRNA-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 4000]
Library Source RNAs extracted from organoids from normal colonic tissue
Cell Lines -
Library Construction (kit name) Single Cell 3′ Library & Gel Bead Kit v2 and the A Chip Kit (10X Genomics)
Fragmentation Methods Enzymatic fragmentation
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 28+91 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000669
Total Data Volume 66.0 GB (fastq)
Comments (Policies) NBDC policy

 

scRNA-seq (JGAS000719)

Participants/Materials

Pancreatic cancer (ICD10: C25): 1 case (6 sample)

 (genetically engineered pancreatic duct organoids)

Targets scRNA-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq 4000]
Library Source RNAs extracted from genetically engineered pancreatic duct organoids
Cell Lines -
Library Construction (kit name) Chromium Next GEM Single Cell 3’ Reagent Kits v3.1
Fragmentation Methods Enzymatic fragmentation
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 28+91 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000852
Total Data Volume 846.3 GB (fastq)
Comments (Policies) NBDC policy

 

ChIP-seq (JGAS000378)

Participants/Materials

Neoplastic disease: 4 cases (5 samples)

    Colon cancer (ICD10: C18)

Targets ChIP-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq X Ten]
Library Source DNAs extracted from organoids derived from colon cancer tissues and a normal epithelial tissue of the patients, and immunoprecipitated with an anti-histone antibody (H3K27Ac)
Cell Lines -
Library Construction (kit name) Illumina Tagment DNA TDE1 Enzyme and Buffer Kits
Fragmentation Methods Ultrasonic fragmentation (Bioruptor Ⅱ)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000492
Total Data Volume 190.5 GB (fastq)
Comments (Policies) NBDC policy

 

 

ChIP-seq (JGAS000719)

Participants/Materials

Pancreatic cancer (ICD10: C25): 5 case (13 sample)

        organoids derived from tumor tissues: 3 cases (3 samples)

        genetically engineered pancreatic duct organoids: 2 cases (10 samples)

Targets ChIP-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq X Ten]
Library Source DNAs extracted from pancreatic cancer organoids or genetically engineered pancreatic duct organoids, and immunoprecipitated with an anti-histone antibody (H3K27me3)
Cell Lines -
Library Construction (kit name) Illumina Tagment DNA TDE1 Enzyme and Buffer Kits
Fragmentation Methods Ultrasonic fragmentation (Bioruptor Ⅱ)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150 bp
QC FRiP score
Mapping Methods Bowtie2
Reference Genome Sequence hg38
Peak Calling Methods (software) MACS2
Japanese Genotype-phenotype Archive Dataset ID JGAD000852
Total Data Volume 882.8 GB (fastq, bed)
Comments (Policies) NBDC policy

 

ATAC-seq

Participants/Materials

Pancreatic cancer (ICD10: C25): 12 cases (16 samples)

        organoids derived from tumor tissues: 10 cases (12 samples)

        genetically engineered pancreatic duct organoids: 2 cases (4 samples)

Targets ATAC-seq
Target Loci for Capture Methods -
Platform Illumina [HiSeq X Ten]
Library Source DNAs extracted from pancreatic cancer organoids or genetically engineered pancreatic duct organoids
Cell Lines -
Library Construction (kit name) Omni-ATAC protocol
Fragmentation Methods Tn5 transposase
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150 x 2 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000852
Total Data Volume 846.3 GB (fastq)
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Toshiro Sato

Affiliation: Department of Organoid Medicine, Keio University School of Medicine

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
Project for Elucidating and Controlling Mechanisms of Aging and Longevity, Japan Agency for Medical Research and Development (AMED) Understanding changes in aging traits aimed at controlling the onset of gastrointestinal diseases JP19gm5010002
Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED) Development of advanced drug discovery system based on understanding of cancer multi-level phenotype JP19cm0106206
Core Research and Evolutional Science and Technology, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-CREST) Dissecting intestinal fibrogenic diseases by a newly developed 4D disease model system JP19gm1210001
KAKENHI Grant-in-Aid for Scientific Research (S) Gaining Integrative Understanding of Gastrointestinal Disease Phenotypes through Establishment of an Organoid Library 17H06176
KAKENHI Grant-in-Aid for Scientific Research (B) Functional analysis of small intestinal epithelial organoid-based transplant graft 20H03746
KAKENHI Grant-in-Aid for Scientific Research (S) Elucidating a role of niche construction in pathophysiological mechanism of human digestive diseases 22H04995

 

PUBLICATIONS

TitleDOIDataset ID
1 Somatic inflammatory gene mutations in human ulcerative colitis epithelium doi: 10.1038/s41586-019-1844-5 JGAD000284
2 An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping doi: 10.1016/j.cell.2020.10.023

JGAD000335

JGAD000336

3 An organoid-based organ repurposing approach to treat short bowel syndrome doi: 10.1038/s41586-021-03247-2 JGAD000359
4 Organoid screening reveals epigenetic vulnerabilities in human colorectal cancer doi: 10.1038/s41589-022-00984-x JGAD000492
5

 

USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use
Klaus H. Kaestner Institue for Diabetes, Obesity & Metabolism at Unversity of Pennsylvania Somatic mutation analysis of the pancreas in type 1 diabetes JGAD000284 2020/04/13-2021/03/03
Ulf Leser Department of Mathematics and Computer Science, Humboldt-Universitaet zu Berlin MAPTor-NET: MAPK-mTOR network model driven individualized therapies of pancreatic neuro-endocrine tumors (pNETs) JGAD000335, JGAD000336 2021/06/03-2023/04/01
Nobuhiro Tanuma Miyagi Cancer Center Research Institute Japan Study on biological characters of pancreatic and gastrointestinal neuroendocrine tumors using patient-derived organoids. JGAD000336 2022/09/19-2023/07/31
Michiaki Hamada Faculty of Science and Engineering, Waseda University Japan Construction of RNA-targeted Drug Discovery Database JGAD000336, JGAD000359, JGAD000492 2022/12/26-2025/03/31