NBDC Research ID: hum0179.v1



Aims: To reveal to what extent long-read data of PromethION deeps our understanding of oral microbiome and virome

Methods: Shutgun metagenome sequencing of bulk DNA extracted from saliva stored in OMNIgeneORAL OM-501 using a enzymatic method

Participants/Materials: Saliva of Japanese healthy individuals


Dataset IDType of DataCriteriaRelease Date
JGAS000186 Metagenome Controlled-access (Type I) 2020/04/03
DRA009794 Metagenome Unrestricted-access 2020/04/03

*Release Note

* Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more

When the research results including the data which were downloaded from NHA/DRA/JGA, are published or presented somewhere, the data user must refer the papers which are related to the data, or include in the acknowledgment. Learn more



JGAS000186 / DRA009794

Participants/Materials: 4 healthy individuals
Targets Metagenome
Target Loci for Capture Methods -

1. Nanopore [PromethION]

2. Illumina [HiSeq]

Library Source DNA extracted from saliva
Cell Lines -
Library Construction (kit name)

1. ligation sequencing kit (SQK-LSK109)

2. Nextera XT

Fragmentation Methods -
Spot Type

1. Single-end

2. Paired-end

Read Length (without Barcodes, Adaptors, Primers, and Linkers)

1. variable, 13kb on average

2. 300 bp

Human Sequence removal Methods EDGE pipeline
DDBJ Sequence Read Archive ID DRA009794
Japanese Genotype-phenotype Archive Dataset ID JGAD000268
Total Data Volume

1. 473 GB (fastq, txt)

2. 186 GB (fastq)

Comments (Policies) NBDC policy

*This study was supported by "Genome Science". "Genome Science" is a part of the "Support Programs for Three Fields in Life Sciences (cancer, genome, and brain sciences)" established by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) under the Grant-in-Aid for Scientific Research on Innovative Areas.



Principal Investigator: Koji Yahara

Affiliation: Antimicrobial Resistance Research Center, National Institute of Infectious Diseases

Project / Group Name: Neo-virology

URL: http://neo-virology.org/en/

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas Big data analysis of bacteriophages and host bacterial using the extended virome datasets and long-read sequencer 19H04846



TitleDOIDataset ID
1 Long-read metagenomics using PromethION uncovers novel oral bacteriophages, their abundance, and interaction with host bacteria doi: 10.1038/s41467-020-20199-9 JGAS000186


USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use