hum0177.v1 Release Note
| Research ID | Release Date | Type of Data |
|---|---|---|
| hum0177.v1 | 2020/04/27 | NGS (PBAT-seq, RNA-seq, ChIP-seq) |
hum0177.v1
- PBAT-seq
DNAs extracted from tumor tissues of malignant glioma and cell line were used for PBAT-sequencing analysis (fastq format files). Libraries were prepared with TACS ligation-mediated PBAT (Miura F, Nucleic Acids Res. 2019) and sequenced using Hiseq X Ten (Illumina).
- RNA-seq
RNAs extracted from tumor tissues of malignant glioma and cell line were used for RNA-sequencing analysis (fastq format files). Libraries were prepared with TruSeq stranded mRNA Kit and sequenced using NovaSeq 6000 (Illumina).
- ChIP-seq
DNAs extracted from cell line of malignant glioma were used for ChIP sequencing analysis (fastq format files). Libraries were prepared with SMARTer ThruPLEX DNA-seq Kit. Anti- H3.3 antibody and anti- H3.3 G34V antibody were used for immunoprecipitation. ChIP-Seq libraries were sequenced using NovaSeq 6000 (Illumina).
Note：
