hum0177.v1 Release Note

Research IDRelease DateType of Data
hum0177.v1 2020/04/27 NGS (PBAT-seq, RNA-seq, ChIP-seq)

 

hum0177.v1

- PBAT-seq

DNAs extracted from tumor tissues of malignant glioma and cell line were used for PBAT-sequencing analysis (fastq format files). Libraries were prepared with TACS ligation-mediated PBAT (Miura F, Nucleic Acids Res. 2019) and sequenced using Hiseq X Ten (Illumina).

 

- RNA-seq

RNAs extracted from tumor tissues of malignant glioma and cell line were used for RNA-sequencing analysis (fastq format files). Libraries were prepared with TruSeq stranded mRNA Kit and sequenced using NovaSeq 6000 (Illumina).

 

- ChIP-seq

DNAs extracted from cell line of malignant glioma were used for ChIP sequencing analysis (fastq format files). Libraries were prepared with SMARTer ThruPLEX DNA-seq Kit. Anti- H3.3 antibody and anti- H3.3 G34V antibody were used for immunoprecipitation. ChIP-Seq libraries were sequenced using NovaSeq 6000 (Illumina).

 

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