NBDC Research ID: hum0176.v1
SUMMARY
Aims: Comprehensive sequencing analyses of uterine and ovarian carcinosarcoma
Methods: Exome / target caputer analysis by using Illumina Hiseq 2000
Participants/Materials: 109 primary tumor and mached normal (non-tumor) tissues from uterin or ovarian carcinosarcoma patients
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000172 | NGS (Exome, Target Capture) | Controlled-access (Type I) | 2019/10/02 |
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MOLECULAR DATA
| Participants/Materials |
Uterine carcinosarcoma (ICD10: C549): 1 case (2 samples) Ovarian carcinosarcoma (ICD10: C56): 3 case (6 samples) |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2000] |
| Library Source | DNAs extracted from tumor and paired non-tumor tissues |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon V4 or V5 |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 101 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000250 |
| Total Data Volume | 646 GB (fastq) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
Uterine carcinosarcoma (ICD10: C549): 91 case (182 samples) Ovarian carcinosarcoma (ICD10: C56): 14 case (28 samples) |
| Targets | Target Capture (CS-A, CS-B) |
| Target Loci for Capture Methods |
CS-A: 491 genes, 2.605 Mbps CS-B: 114 genes, 422.9 kbps |
| Platform | Illumina [HiSeq 2000] |
| Library Source | DNAs extracted from tumor and paired non-tumor tissues |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect XT Custom |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 101 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000250 |
| Total Data Volume | 646 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Seiichi Mori
Affiliation: Cancer Precison Medicine Center
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| The Vehicle Racing Commemorative Foundation | Exploration of relevant mutations associated with relapsing or refractory ovarian cancer. | 5144, 5274, and 5393 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | Genome-wide analyses of endometrial endometrioid carcinoma for early detection and personalized medicine. | 26462543 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | Molecular analysis of malignant ascites formation by single-cell RNA-sequencing. | 15K06861 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | Identification of the progestin therapy resistance genes in time-course endometrial cancer samples. | 17K11308 |
| KAKENHI Grant-in-Aid for Young Scientists (B) | Whole genome sequencing analyses of uterine and ovarian carcinosarcoma. | 17K18337 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | Immune microenvironment analyses of uterine and ovarian carcinosarcoma. | 18K07338 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | |||
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
| Masaki Mandai | Kyoto University Faculty of Medicene, department of Gynecology and Obstetrics | Elucidation of immune-system networks between host and tumor based on genomic analysis | JGAD000250 | 2020/03/13-2025/03/31 | |
| Kouya Shiraishi | Division of Genome Biology, National Cancer Research Institute | Integrated analyses of omics (genomics, transcriptomics, proteomics and metabolomics) associated with clinical variables for developing indivisualizedtreatment in gynecological malignancy | JGAD000250 | 2020/09/18-2023/03/31 |
