NBDC Research ID: hum0168.v1

SUMMARY

Aims: Analysis of genetic background of adenomyosis

Methods: Whole exome sequencing was performed on fresh fronzen samples from adenomyosis individuals

Participants/Materials: 51 patients of adenomyosis

Dataset ID	Type of Data	Criteria	Release Date
JGAS000169	NGS (Exome)	Controlled-access (Type I)	2019/11/08

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MOLECULAR DATA


Participants/Materials	adenomyosis: 51 cases
Targets	Exome
Target Loci for Capture Methods	-
Platform	Illumina [HiSeq 2500]
Library Source	DNAs extracted from fresh frozen samples (adenomyosis, co-occurring endometriosis, co-occurring leiomyoma, co-occurring ovarian cancer, normal myometrium, monocytic cells in ascitic fluid, peripheral blood cells) from adenomyosis individuals
Cell Lines	-
Library Construction (kit name)	NEBNext Ultra DNA library prep kit for Illumina, Agilent V6 plus custom probes
Fragmentation Methods	Ultrasonic fragmentation (Covaris)
Spot Type	Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	125 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000247
Total Data Volume	2 TB (bam [ref: hg38])
Comments (Policies)	NBDC policy

Principal Investigator: Masahito Kawazu

Affiliation: National Cancer Center Research Institute

Project / Group Name: Division of Cellular Signaling

Funds / Grants (Research Project Number):

Name	Title	Project Number
KAKENHI Grant-in-Aid for Scientific Research (C)	Establishment of highly sensitive SNV detection method of WES for uterine adenomyosis by LCM method.	19K07708

	Title	DOI	Dataset ID
1	Uterine adenomyosis is an oligoclonal disorder associated with KRAS mutations	doi: 10.1038/s41467-019-13708-y	JGAD000247
2

Principal Investigator	Affiliation	Country/Region	Research Title	Data in Use (Dataset ID)	Period of Data Use