NBDC Research ID: hum0146.v1
SUMMARY
Aims: To investigate DNA methylation change in Silver-Russell Syndrome (SRS) patients with assisted reproduction technologies (ART)
Methods: Reduced Representation Bisulfite Sequencing (RRBS)
Participants/Materials: SRS patients born after assisted reproduction technologies (ART), spontaneously conceived SRS patients, and spontaneously conceived children
URL: http://www.med.tohoku.ac.jp/english/about/laboratory/101.html
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| DRA007187 | NGS (RRBS) | Unrestricted-access | 2019/02/19 |
*When the research results including the data which were downloaded from DRA database, are published or presented somewhere, the data user must refer the papers which are originally reported, or include in the acknowledgment. Example
MOLECULAR DATA
| Participants/Materials: |
5 SRS patients born after ART (ICD10: Q871) 5 spontaneously conceived SRS patients (ICD10: Q871) 10 spontaneously conceived children |
| Targets | RRBS |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2500] |
| Library Source | DNA extracted from peripheral blood cells |
| Cell Lines | - |
| Library Construction (kit name) | Reduced representation bisulfite sequncing (RRBS) |
| Fragmentation Methods | Enzymatic fragmentation (MspI) |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 101 bp (includes adaptors) |
| Calculation for Methylation Rate | Bismark methylation extractor (v.0.9.0) |
| DDBJ Sequence Read Archive ID | DRA007187 |
| Total Data Volume | 38.5 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Takahiro Arima
Affiliation: Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Practical Research Project for Rare / Intractable Diseases, Japan Agency for Medical Research and Development (AMED) | Molecular diagnosis of congenital imprinting disorders and associated with assisted reproductive technology (ART) | JP17ek0109132h003 |
| KAKENHI Grant-in-Aid for Scientific Research (B) | Epigenetic cohort study in the ART-derived children | 17H04335 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Association of four imprinting disorders and ART. | doi: 10.1186/s13148-019-0623-3 | DRA007187 |
| 2 |
