NBDC Research ID: hum0143.v1
SUMMARY
Aims: The aim of the present study is to elucidate whether aberrant (P)RR expression induces genomic instability in human pancreatic ductal epithelial (HPDE) cells, which are transforming into pancreatic ductal adenocarcinoma (PDAC).
Methods: Comparative analysis of the human whole genome between HPDE cells overexpressed by(pro)renin receptor and control HPDE cells
Participants/Materials: Immortalized human pancreatic ductal epithelial (HPDE) cells
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000143 | NGS (WGS) | Controlled-access (Type I) | 2025/05/12 |
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MOLECULAR DATA
| Participants/Materials |
HPDE cells (P)RR-expressing (persistent) HPDE-1/E6E7 cells: 1 sample (P)RR-expressing (transient) HPDE-1/E6E7 cells: 1 sample Mock-expressing (persistent) HPDE-1/E6E7 cells: 1 sample Mock-expressing (transient) HPDE-1/E6E7 cells: 1 sample HPDE-1/E6E7 cells: 1 sample HPDE-6/E6E7 cells: 1 sample |
| Targets | WGS |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2500, Novaseq 6000] |
| Library Source | DNA extracted from cell lines |
| Cell Lines | HPDE-1/E6E7, HPDE-6/E6E7 |
| Library Construction (kit name) | TruSeq DNA PCR-Free LT Library Prep Kit |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 125 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000212 |
| Total Data Volume | 410 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Akira Nishiyama
Affiliation: Department of Pharmacology, Faculty of Medicine, Kagawa Universty
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (B) | Kidney-mediated aging process | 18H03191 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | Evolutionary machanism of pancreatic ductal adenocarcinoma mediated by (pro)renin receptor | 19K07690 |
| KAKENHI Grant-in-Aid for challenging Exploratory Research | Functional analysis of (pro)renin receptor in the genesis of pancreatic cancer | 16K14610 |
| Hoansha Foundation and Alumni Association of Kagawa University (Sanjukai) | Development of new therapeutic agents for lifestyle-related diseases | - |
| TOBIRA, Tokyo Biomarker Innovation Research Associate | Examination of the availability of soluble (pro)renin receptor as an early marker of pancreatic cancer | - |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Aberrant (pro)renin receptor expression induces genomic instability in pancreatic ductal adenocarcinoma through upregulation of SMARCA5/SNF2H | doi: 10.1038/s42003-020-01434-x | JGAD000212 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
