NBDC Research ID: hum0141.v1
SUMMARY
Aims: Analysis of immune regulation in head and neck cancer
Methods: RNA-seq
Participants/Materials: Head and neck cancer
URL: http://www.med.nagoya-cu.ac.jp/immunol.dir/new1.html
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000135 | NGS (RNA-seq) | Controlled-access (Type I) | 2019/05/09 |
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MOLECULAR DATA
| Participants/Materials |
Head and neck cancer: 4cases (15 samples) HNC1: Malignant neoplasm of supraglottis (ICD10: C32.1) HNC10: Malignant neoplasm of base of tongue (ICD10: C01) HNC14: Malignant neoplasm of other and ill-defined sites in the lip, oral cavity and pharynx (ICD10: C12) HNC18: Malignant neoplasm of posterior wall of hypopharynx (ICD10: C13.2) |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Thermo Fisher Scientific [Ion S5] |
| Library Source |
RNAs extracted from isolated cells using different markers from tumor tissues of head and neck cancer - Double Positive (DP): CD25+ CTLA4+ Treg cells - CD25: CD25+ T cells - CTLA4: CTLA4+ T cells - Double Negative (DN): CTLA4- CD25- T cells |
| Cell Lines | - |
| Library Construction (kit name) | KAPA Library Preparation Kit |
| Fragmentation Methods | KAPA Frag |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 150 bp on average |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000204 |
| Total Data Volume | 70 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Shimon Sakaguchi
Affiliation: Experimental Immunology, IFReC, Osaka University
Project / Group Name: Department of Immunology, Nagoya City University Graduate School of Medical Sciences
URL: http://www.med.nagoya-cu.ac.jp/immunol.dir/new1.html
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for challenging Exploratory Research | Investigating the mechanisms of regulatory T cell expansion by aging | 26670192 |
| KAKENHI Grant-in-Aid for challenging Exploratory Research | Investigating regulatory T cells in skin rare cancer to predict effectiveness of a new immunotherapy | 16K15259 |
| KAKENHI Grant-in-Aid for Scientific Research (B) | Research on regulatory T cell-therapy without inducing cancer and severe infections-the safe and effective immunosuppression from Japan | 16H05177 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | Developing C5a antagonists specific for oral cancer and investigating mechanisms of cancer proliferation (M.I.) | 17K11679 |
| KAKENHI Grant-in-Aid for Young Scientists (B) | Investigating roles of regulatory T cells in inducing anti-tumur immunity and developing immunotherapy in head and neck cancer (T.M.) | 17K16937 |
| KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) | Developing a new therapy for autoimmnue dieseases using regulatory T cell and neo-self induced by ultraviolet | 17H05798 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Regulatory T cells expressing abundant CTLA-4 on the cell surface with a proliferative gene profile are key features of human head and neck cancer. | doi: 10.1002/ijc.32024 | JGAD000204 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
| Robert Tsai | Institute of Biosciences & Technology, Texas A&M Health Science Center | Genome-wide Discovery of new Plasma DNA Mehylation Makers for Tracking the Progression of NAFLD to NASH and HCC | JGAD000204 | 2020/11/06-2022/08/31 | |
| Michiaki Hamada | Faculty of Science and Engineering, Waseda University | Japan | Construction of RNA-targeted Drug Discovery Database | JGAD000204 | 2022/12/26-2025/03/31 |
