hum0116 Release Note

hum0116.v1

- DNAs extracted from leukemia stem cells, pre-leukemia stem cells, T-cell, B-cells, and leukemia stem cells from humanized mice were used for the Target Capture sequencing analysis (fastq files). Libraries were prepared by using of KAPA Hyper Prep kit and xGen® Acute Myeloid Leukemia Cancer Panel and sequenced by HiSeq 1500 (single-end: 50 bp).

- DNAs extracted from peripheral blood, bone marrow aspirate from AML patients and human leukemia cells from spleen and bone marrow aspirate of humanized mice with leukemia derived from immune deficiency mice which were infused with peripheral blood, bone marrow aspirate or samples from patients with AML were used for the Target Capture sequencing analysis (fastq files). 1st PCR was performed to amplify target regions from blood derived or manipulated DNAs, followed by the 2nd PCR to add 8-bp barcode and adapter sequences. Then, DNA libraries were sequenced by HiSeq 2500 (paired-end).

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