hum0111 Release Note
| Research ID | Release Date | Type of Data |
|---|---|---|
| hum0111.v3 | 2021/02/19 | NGS (Amplicon-seq) |
| hum0111.v2 | 2019/05/31 | NGS (Amplicon-seq) |
| hum0111.v1 | 2017/07/31 | NGS (RNA-seq, Amplicon-seq) |
hum0111.v3
RNAs were extracted from tumor and normal tissues of 102 gastric carcinoma patients. Libraries for Amplicon-seq (V region to C region of IgVH) were prepared by PCR and Amplicon-seq was performed by Illumina Miseq (300 bp x 2, Paired-end). The sequences are provided as fastq files.
hum0111.v2
RNAs were extracted from tumor and normal tissues of 60 gastric carcinoma patients. Libraries for Amplicon-seq (V region to C region of IgVH) were prepared by PCR and Amplicon-seq was performed by Illumina Miseq (300 bp x 2, Paired-end). The sequences are provided as fastq files.
hum0111.v1
RNAs were extracted from tumor and normal tissues of 30 diffuse-type gastric carcinoma patients. Libraries for RNA-seq were prepared using TruSeq Stranded mRNA Sample Prep Kit and a deep sequencing was performed by using of Illumina HiSeq 2000 (100 bp x 2, Paired-end). The FPKM（Fragments Per Kilobase of transcript per Million mapped fragments）values are provided as a txt file. Libraries for Amplicon-seq (V region to C region of TCRα, TCRβ, IgVH and IgVκ/λ) were prepared by PCR and Amplicon-seq was performed by Illumina Miseq (300 bp x 2, Paired-end). The sequences are provided as fastq files.
Note:
