hum0086 Release Note

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hum0086.v1

NGS (WGBS)

DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for WGBS analyses (fastq format files). Libraries were prepared by Post-Bisulfite Adaptor-Tagging (PBAT) method and sequencing was performed with Illumina [HiSeq 1500 / 2500] (101 bp, single-end / paired-end).

NGS (ChIP-seq)

DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for ChIP-seq analyses (fastq format files). Anti H3K4me3, H3K4me1, H3K9me3, H3K27ac, H3K27me3, H3K36me3 antibodies were used for immunoprecipitation. ChIP-Seq libraries were prepared by using Ovation Ultralow System V2 kit (NuGEN) and NEBNext UltraII DNA Library Prep Kit for Illumina and sequenced using Illumina [HiSeq 2500] (51 - 126 bp, single-end / paired-end).

NGS (RNA-seq)

mRNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for RNA-seq analyses (fastq format files). Libraries were prepared by using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequencing was performed with Illumina [HiSeq 2500] (101-126 bp, paired-end).

NGS (small RNA-seq)

RNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for small RNA-seq analyses (fastq format files). Libraries were prepared by using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) and sequencing was performed with Illumina [HiSeq 1500 / 2500] (64 / 68 bp [including adaptor], single-end).

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