hum0086 Release Note
| Research ID | Release Date | Type of Data |
|---|---|---|
|
2018/06/26 2017/12/26 2017/09/25 |
NGS (PBAT-seq) NGS (ChIP-seq) NGS (RNA-seq) NGS (small RNA-seq) |
hum0086.v3
Data addition of
- RNA-seq data for placental stroma
- PBAT-seq, RNA-seq, and small RNA-seq data for cytotrophoblast (fetal growth restriction, preeclampsia, trisomy 18)
- PBAT-seq data for hydatidiform mole
hum0086.v2
Data addition of
- PBAT-seq, ChIP-seq, RNA-seq, and small RNA-seq data for female trophoblast stem cells (TS) derived from CT, ST differentiated from TS, EVT differentiated from TS
- PBAT-seq data for male TS derived from CT
- RNA-seq data for male TS derived from CT, ST differentiated from TS, and EVT differentiated from TS
- ChIP-seq data for placental stroma
hum0086.v1
NGS (PBAT-seq)
DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for PBAT-seq analyses (fastq format files). Libraries were prepared by Post-Bisulfite Adaptor-Tagging (PBAT) method and sequencing was performed with Illumina [HiSeq 1500 / 2500] (101 bp, single-end / paired-end).
NGS (ChIP-seq)
DNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for ChIP-seq analyses (fastq format files). Anti H3K4me3, H3K4me1, H3K9me3, H3K27ac, H3K27me3, H3K36me3 antibodies were used for immunoprecipitation. ChIP-Seq libraries were prepared by using Ovation Ultralow System V2 kit (NuGEN) and NEBNext UltraII DNA Library Prep Kit for Illumina and sequenced using Illumina [HiSeq 2500] (51 - 126 bp, single-end / paired-end).
NGS (RNA-seq)
mRNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for RNA-seq analyses (fastq format files). Libraries were prepared by using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequencing was performed with Illumina [HiSeq 2500] (101-126 bp, paired-end).
NGS (small RNA-seq)
RNAs extracted from placenta (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast) and uterus (endometrium epitherial, stroma) were used for small RNA-seq analyses (fastq format files). Libraries were prepared by using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) and sequencing was performed with Illumina [HiSeq 1500 / 2500] (64 / 68 bp [including adaptor], single-end).
Note:
