NBDC Research ID: hum0060.v1



Aims: To find the characteristics of recurrent / metastatic breast cancers by carrying out a "molecular profiling", mainly the exome sequence analysis of cancerous tissues of the primary and recurrence tumors from the same patient with recurrent / metastatic breast cancer.

Methods: Whole exome sequencing by using of Illumina HiSeq 2000

Participants/Materials: Total of 175 normal, primary tumor, and metastasis tumor samples from 65 breast cancer patients


Dataset IDType of DataCriteriaRelease Date
JGAS000075 NGS (Exome) Controlled-access (Type I) 2018/04/03

*Release Note 

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Participants/Materials 65 patient with recurrent / metastatic breast cancer
Targets Exome
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2000]
Library Source DNAs extracted from normal, primary tumor and metastasis tumor tissues
Cell Lines -
Library Construction (kit name) SureSelect Human All Exon V5 + lincRNA
Fragmentation Methods Ultrasonic fragmentation (Covaris E220)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 100 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000075
Total Data Volume 4 TB (fastq [350 files])
Comments (Policies) NBDC policy



Principal Investigator: Yoshio Miki

Affiliation: Medical Research Institute, Tokyo Medical and Dental University

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT), Japan Agency for Medical Research and Development (AMED) Development of the Intractable Cancer Therapies through the New Target Identification by the Molecular Profiling



TitleDOIDataset ID


USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use
Masaki Mandai Kyoto University Faculty of Medicene, department of Gynecology and Obstetrics Integrated analyses of omics (genomics, transcriptomics, proteomics and metabolomics) associated with clinical variables for developing indivisualizedtreatment in gynecological malignancy JGAD000075 2020/03/13-2025/03/31
Takashi Kohno Division of Genome Biology, National Cancer Center Research Institute Identification of genetic risk factors in AYA (Adolescence and Young Adult) cancer JGAD000075 2021/09/27-2025/03/31