NBDC Research ID: hum0050.v1

SUMMARY

Aims: Understand blood transcriptome of HBV positive blood.

Methods: Extract RNA from whole blood and prepare CAGE libraries for deep sequencing

Participants/Materials: HBV (Genotype C) positive blood samples derived from untreated patients (men)

Dataset ID	Type of Data	Criteria	Release Date
JGAS000053	NGS (CAGE-seq)	Controlled-access (Type I)	2016/09/30

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MOLECULAR DATA


Participants/Materials	8 HBV (Genotype C) positive blood samples derived from untreated patients (men)
Targets	CAGE-seq
Target Loci for Capture Methods	-
Platform	Illumina [HiSeq 2000]
Library Source	RNA extracted from whole blood samples
Cell Lines	-
Library Construction (kit name)	nAnT-iCAGE v2.1
Fragmentation Methods	-
Spot Type	Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	47 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000053
Total Data Volume	3 GB (fastq, bam [ref: hg19])
Comments (Policies)	NBDC policy

Principal Investigator: Tomokazu Matsuura

Affiliation: The Jikei University School of Medicine, Rinsho Kensa Igaku Koza

Project / Group Name: -

Funds / Grants (Research Project Number):

Name	Title	Project Number
Division of Infectious Disease Research, Research Program on Hepatitis, Japan Agency for Medical Research and Development (AMED)	Drug Discovery Research for Complete Cure of Chronic Hepatitis Type B Utilizing The Next Generation Life Science Technologies	JP15fk0310004

	Title	DOI	Dataset ID
1	Single-nucleotide-resolution mapping of HBV promoters in infected human livers and hepatocellular carcinoma	doi: 10.1128/JVI.01625-16	JGAS000053
2

Principal Investigator	Affiliation	Country/Region	Research Title	Data in Use (Dataset ID)	Period of Data Use