NBDC Research ID: hum0027.v1

 

SUMMARY

Aims: To identify polymorphisms (SNP and Indel) of responsible genes for Japanese aggressive periodontitis by whole exon sequencing (WES) analysis. Then, functional analyses forf the responsible genes were assessed by in vivo/vitro biological experiments.

Methods: WES by using of HiSeq 2000 (Illumina).

Materials: 44 Japanese aggressive periodontitis patients

 

Dataset IDType of DataCriteriaRelease Date

JGAS000024

JGAS000040

 NGS (Exome) Controlled-access (Type I) 2016/07/28

*Release Note

*Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more

 

MOLECULAR DATA

JGAS000024 / JGAS000040
Materials 44 Japanese aggressive periodontitis patients
Targets Exome
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2000]
Library Source gDNA extracted from peripheral blood cells
Cell Lines -
Library Construction (kit name) Agilent SureSelect Human All Exon V5 (50M) kit
Fragmentation Methods Ultrasonic fragmentation (Covaris E220)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150 bp
Japanese Genotype-phenotype Archive Dataset ID

JGAD000024 （hg18）

JGAD000040 （hg19）
Total Data Volume 460 GB (fastq, bam, vcf)
Comments (Policies)

NBDC policy

Academic Use Only (till 2017/07/28)

 

DATA PROVIDER

Principal Investigator: Shinya Murakami

Affiliation: Division of Oral Biology and Disease Control, Department of Periodontology, Osaka University Graduate School of Dentistry

Project / Groupe Name:

URL: http://www.dent.osaka-u.ac.jp/english/g_school/facilities/periodontology.html

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research (A) Identification of genetic risk factors on aggressive periodontitis in a Japanese population 15H02579
KAKENHI Grant-in-Aid for Scientific Research (C) Effects of cathepsin A on the regeneration of periodontal tissue 15K11385

 

PUBLICATIONS

TitleDOIDataset ID
1 - - -