NBDC Research ID: hum0011.v1



Aims: To clarify integrated HBV sequences in the human genome.

Methods: Target enrichment sysem-based next generation sequencer

Participants/Materials: PLC/PRF/5 cell, HepG22215 cell, and HB-HCC primary tissues


Dataset IDType of DataCriteriaRelease Date
JGAS000015 NGS (Target Capture) Controlled-access (Type I) 2015/10/07

*Release Note

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1 PLC/PRF/5 cell

1 HepG22215 cell

8 HB-HCC primary tissues

Targets Target Capture
Target Loci for Capture Methods hepatitis B viral integrants with flanking human genomic sequences
Platform Roche GS FLX Titanium
Library Source HB-HCC primary tissues
Cell Lines

PLC/PRF/5 cell [hepatocellular carcinoma cell line (producing HBsAg)]

HepG22215 cell [hepatoblastoma cell line (contain an integrated copy of the HBV genome)]

Library Construction (kit name) custom
Fragmentation Methods Ultrasonic fragmentation (Covaris)
Spot Type GS Run Processor
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 321.10 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000015
Total Data Volume 1.84 GB
Comments (Policies) NBDC policy



Principal Investigator: Hiroyuki Yamamoto

Affiliation: St. Marianna University School of Medicine, Division of Gastroenterology and Hepatology

Project / Group Name:

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research (C) Sequence analysis at HBV integrants and flanking host genomes 23590964



TitleDOIDataset ID
1 DNA methylation at hepatitis B viral integrants is associated with methylation at flanking human genomic sequences. doi: 10.1101/gr.175240.114. JGAD000015


USERS (Controlled-access Data)

Principal Investigator: Affiliation: Data in Use (Dataset ID)Period of Data Use