NBDC Research ID: hum0006.v4
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SUMMARY
Aims: The identification of genomic/genetic alterations in brain tumors (gliomas) and analysis of their clinicopathological significance.
Methods: Exome sequencing, RNA sequencing and Methylation array were performed. Matched normal DNA was also used for Exome sequencing.
Participants/Materials:
Surgical specimen obtained from 6 patients with astrocytoma (primary and matched recurrent samples) and paired normal peripheral blood cells (total: 23 samples).
Surgical specimen obtained from 16 patients with oligodendroglioma (12 primary and matched recurrent samples, 8 samples obtained from 4 patients by multi-sampling)
Surgical specimen obtained from patients with gliomas (17 patients with diffuse cerebellar glioma, 8 patients with cerebral glioblastoma and 14 patients with thalamic glioma)
Surgical specimen obtained from 94 patients with gliomas, including primary and matched recurrent samples (total 114 samples) and paired normal blood cells
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000004 |
astrocytoma NGS (Exome) |
Controlled-access (Type I) | 2014/01/31 |
| JGAS000004 data addition |
oligodendroglioma NGS (Exome, RNA-seq), Methylation array |
Controlled-access (Type I) | 2017/06/05 |
| JGAS000106 |
gliomas (diffuse cerebellar glioma, cerebral glioblastoma, thalamic glioma) NGS (Exome, RNA-seq), Methylation array |
Controlled-access (Type I) | 2017/09/01 |
| JGAS000146 |
gliomas NGS (Exome, RNA-seq), Methylation array |
Controlled-access (Type I) | 2019/02/13 |
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MOLECULAR DATA
| Participants/Materials |
astrocytoma (ICD10: C71): 6 cases (23 samples) oligodendroglioma (ICD10: C71): 16 cases (48 samples) diffuse cerebellar glioma (ICD10: C71): 17 cases (34 samples) glioma (ICD10: C71): 12 cases (25 tumor samples, 12 non-tumor samples) 11 pairs of primary-recurrent gliomas and 1 primary glioma |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2000] |
| Library Source | DNAs extracted from surgical specimen of brain tumors (primary and matched recurrent samples) and paired normal peripheral blood cells. |
| Cell Lines | - |
| Library Construction (kit name) | Agilent SureSelect Human All Exon v.4 |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris SS) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID |
JGAD000004 (astrocytoma) JGAD000106 (oligodendroglioma) JGAD000112 (diffuse cerebellar glioma) JGAD000215 (primary grade II/III gliomas and their recurrent tumors; 36 samples, matched normal blood; 16 samples) JGAD000218 (primary grade IV gliomas and their recurrent tumors; 16 samples, matched normal blood; 8 samples) |
| Total Data Volume |
JGAD000004: 375.31 GB (fastq, bam [reference: hg19]) JGAD000106: 885.30 GB (fastq, bam [reference: hg19]) JGAD000112: 506.99 GB (fastq, bam [reference: hg19]) JGAD000215: 1.32 TB (fastq, bam [reference: hg19]) JGAD000218: 552.72 GB (fastq, bam [reference: hg19]) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
oligodendroglioma (ICD10: C71): 16 cases (27 samples) diffuse cerebellar glioma (ICD10: C71): 14 cases (14 samples) cerebral glioblastoma (ICD10: C71): 8 cases (8 samples) glioma (ICD10: C71): 24 cases (31 samples) 8 pairs of primary-recurrent gliomas, 8 primary gliomas, and 7 recurrent gliomas |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2000] |
| Library Source | RNAs extracted from surgical specimen of brain tumors (primary and matched recurrent samples) and paired normal peripheral blood cells. |
| Cell Lines | - |
| Library Construction (kit name) | TruSeq Stranded Total RNA Library Prep Kit |
| Fragmentation Methods | Alkalization |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID |
JGAD000113 (diffuse cerebellar glioma, cerebral glioblastoma) JGAD000216 (gliomas including primary grade II/III gliomas and their recurrent tumors; 31 samples) JGAD000219 (recurrent tumors of primary grade IV gliomas; 8 samples) |
| Total Data Volume |
JGAD000107: 239.26 GB (bam, fastq） JGAD000113: 363.23 GB (bam, fastq） JGAD000216: 302.43 GB (bam, fastq） JGAD000219: 96.94 GB (bam, fastq） |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
oligodendroglioma (ICD10: C71): 16 cases (30 samples) diffuse cerebellar glioma (ICD10: C71): 17 cases (17 samples) thalamic glioma (ICD10: C71): 14 cases (14 samples) glioma (ICD10: C71): 93 cases (113 samples) 20 pairs of primary-recurrent gliomas, 69 primary gliomas, and 4 recurrent gliomas |
| Targets | Methylation array |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HumanMethylation 450 BeadChip] |
| Library Source | DNAs extracted from surgical specimen of brain tumors (primary and matched recurrent samples) and paired normal peripheral blood cells. |
| Cell Lines | - |
| Library Construction (kit name) | Illumina HumanMethylation450 BeadChip kit |
| Algorithms for Calculating Methylation-rate (software) | Genome Studio v2010.3 |
| Filtering Methods | HumanMethylation450_15017482_v1.1 (bpm) |
| Probe Number (after QC) | 485577 methylation sites |
| Japanese Genotype-phenotype Archive Dataset ID |
JGAD000114 (diffuse cerebellar glioma, thalamic glioma) JGAD000217 (gliomas including primary grade II/III tumors and their recurrent tumors; 113 samples) |
| Total Data Volume |
JGAD000108: 139.75 MB (txt) JGAD000114: 154.82 MB (txt) JGAD000217: 398.82 MB (txt) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Nobuhito Saito
Affiliation: Department of Neurosurgery, The University of Tokyo
Project / Group Name: Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT)
URL: http://p-direct.jfcr.or.jp/english/
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT), Japan Agency for Medical Research and Development (AMED) | Development of novel therapeutic strategy and biomarker for the treatment of malignant brain tumors | - |
| Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED) | Analysis of the mechanisms for development of tumor heterogeneity related to treatment resistance of gliomas and discovery of therapeutic strategies | - |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Mutational Analysis Reveals the Origin and Therapy-Driven Evolution of Recurrent Glioma | doi:10.1126/science.1239947 | JGAD000004 |
| 2 | Genetic and epigenetic stability of oligodendrogliomas at recurrence | doi: 10.1186/s40478-017-0422-z | JGAD000106-JGAD000108 |
| 3 | Distinct molecular profile of diffuse cerebellar gliomas | doi: 10.1007/s00401-017-1771-1 | JGAD000112-JGAD000113 |
| 4 | DNA demethylation is associated with malignant progression of lower-grade gliomas | doi: 10.1038/s41598-019-38510-0 | JGAD000215-JGAD000219 |
USERS (Controlled-access Data)
| Principal Investigator: | Affiliation: | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|
