hum0006 Release Note
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| Research ID | Release Date | Type of Data |
|---|---|---|
| hum0006.v4 | 2019/02/13 | NGS (Exome, RNA-seq), Methylation array |
| hum0006.v3 | 2017/09/01 | NGS (Exome, RNA-seq), Methylation array |
| hum0006.v2 | 2017/06/05 | NGS (Exome, RNA-seq), Methylation array |
| hum0006.v1 | 2014/01/31 | NGS (Exome) |
hum0006.v4
Analysis data about 94 gliomas (114 samples) were added (JGAS000146).
• Exome (JGAD000215, JGAD000218) : glioma: 12 cases (25 tumor samples, 12 non-tumor samples)
DNAs extracted from gliomas and peripheral blood were used for the whole exome sequencing. Exons were narrowed down by using of SureSelect Human All Exon v.4 and read by Illumina HiSeq 2000 (paired-end: 100 bp)
• RNA-seq (JGAD000216, JGAD000219) : glioma: 23 cases (31 samples)
RNAs extracted from gliomas were used for RNA-seq. RNA liberally builded using TruSeq Stranded Total RNA Library Prep Kit and read by Illumina HiSeq 2000 (paired-end: 100 bp)
• Methylation array (JGAD000217) : glioma: 93 cases (113 samples)
DNAs extracted from gliomas were performed with methylation analysis using Illumina HumanMethylation 450 BeadChip.
hum0006.v3
Analysis data about 39 gliomas (17 diffuse cerebellar gliomas, 14 cerebral glioblastomas, 8 thalamic gliomas) were added (JGAS000106).
• Exome: diffuse cerebellar glioma (ICD10:C71): 17 cases (34 samples)
DNAs extracted from brain tumors (diffuse cerebellar gliomas, cerebral glioblastomas, thalamic gliomas) and peripheral blood were used for the whole exome sequencing. Exons were narrowed down by using of SureSelect Human All Exon v.4 and read by Illumina HiSeq 2000 (paired-end: 100 bp)
• RNA-seq: diffuse cerebellar glioma: 14 cases (14 samples), cerebral glioblastoma: 8 cases (8 samples)
RNAs extracted from brain tumors (diffuse cerebellar glioma, cerebral glioblastoma) were used for RNA-seq (bam and fastq). RNA liberally builded using TruSeq Stranded Total RNA Library Prep Kit and read by Illumina HiSeq 2000 (paired-end: 100 bp)
• Methylation array: diffuse cerebellar glioma: 17 cases (17 samples), thalamic glioma: 14 cases (14 samples)
DNAs extracted from brain tumors (diffuse cerebellar glioma, thalamic glioma) were performed with methylation analysis using Illumina HumanMethylation 450 BeadChip.
hum0006.v2
Analysis data about 16 oligodendrogliomas were added to JGAS000004.
• Exome: 48 samples
DNAs extracted from brain tumors (oligodendrogliomas) and peripheral blood were used for the whole exome sequencing. Exons were narrowed down by using of SureSelect Human All Exon v.4 and read by Illumina HiSeq 2000 (paired-end: 100 bp)
• RNA-seq: 27 samples
RNAs extracted from brain tumors (oligodendrogliomas) were used for RNA-seq (bam and fastq). RNA liberally builded using TruSeq Stranded Total RNA Library Prep Kit and read by Illumina HiSeq 2000 (paired-end: 100 bp)
• Methylation array: 30 samples
DNAs extracted from brain tumors (oligodendrogliomas) were performed with methylation analysis using Illumina HumanMethylation 450 BeadChip.
hum0006.v1
DNAs extracted from brain tumors (6 astrocytomas) and peripheral blood were used for the whole exome sequencing (bam files). Exons were narrowed down by using of SureSelect Human All Exon v.4 and read by Illumina HiSeq 2000 (paired-end: 100 bp)
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