Dataset ID
JGAD000805
- Type of data
- NGS (RNA-seq)
- Access criteria
- Controlled-access (Type I)
- Total data volume
- 117 GB
- File formats
- FASTQ
- Research
- hum0442
- Date published
- 2024-08-09
- Date modified
- 2024-12-06
- DDBJ Search
- JGAD000805 (opens in a new tab)
- JGA Study
- JGAS000675 (opens in a new tab)
Analysis method
RNA-seq
- Materials and participants
- advanced or recurrent non-small cell lung cancer (ICD10: C349) before administering anti-PD-1/PD-L1 antibody treatment: 20 cases
FFPE tumor tissue: 20 samples - Health statusAffected
- Subject count20 (Individual)
- Disease
- advanced or recurrent non-small cell lung cancer (C349)
- Sample description
- RNAs extracted from tumor tissues
- Tumor / normalTumor
- Sample provider
- N/A
- Experimental method
- RNA-seq
- Target
- N/A
- Reagent kit
- Nextera XT DNA Library Prep Kit
SMART-Seq Stranded Kit - Fragmentation
- Nextera XT DNA Library Prep Kit
- Platform
- Illumina NovaSeq 6000
- Read type
- Paired-end
- Read length
- 150 bp
- Reference genome
- GRCh37
GRCh38 - Mapping
- The count data was mapped to the reference genome using STAR aligner for calculation.
- QC and filtering
- Read count and TPM data calculated after adapter trimming of fastq files.
- Analysis method
- HTSeq
- Gene count
- Count data: 25,559
TPM data: 14,222 - Data use policy
- NBDC data sharing policy (JGAP000001)