Skip to content
NBDC Human Database

No datasets in the cart.

Go to the cart

Due to system maintenance, the application system, application review by the Data Access Committee will be unavailable during the following period.
Schedule: October 5th (Mon), 2026, 9:00 - October 7th (Wed), 2026, 15:00 (JST)
We apologize for any inconvenience this may cause and appreciate your understanding.

We are currently receiving a large number of applications for data submission, and the review process is taking longer than usual.We sincerely apologize for the delay and kindly ask for your understanding. When submitting an application, we would greatly appreciate it if you could allow sufficient time for the processing.

Following a change to our organizational structure effective April 1, 2026, this division has been renamed from the "Database Center for Life Science, Joint Support-Center for Data Science Research" to the "Database Division for Life Science (DBCLS), BioData Science Initiative (BSI), National Institute of Genetics (NIG)". Where the former name still appears in the guidelines, please read it as the new name.

Dataset ID

E-GEAD-1108

Type of data
NGS (scRNA-seq, scTCR-seq, scCITE-seq) processed data
Access criteria
Unrestricted-access
Total data volume
83.8 MB
File formats
  • TXT
  • TAR
Research
hum0511
Date published
2026-07-03
Date modified
2026-09-11
DDBJ Search
E-GEAD-1108 (opens in a new tab)

Analysis method

scRNA-seq

Materials and participants
6 healthy individuals (JGAD000957/Dataset 2)
70s-90s: 2
100s: 2
110s: 2
  • Health status
    Healthy
  • Subject count
    6 (Individual)
  • Age group
    Elderly
Sample description
RNAs extracted from peripheral blood T cells stimulated for 6 hours with PMA and ionomycin
  • Tissue
    Peripheral blood, T cell
Sample provider
N/A
Experimental method
scRNA-seq
Target
N/A
Reagent kit
Chromium Next GEM Single Cell 5' v2
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
72 bp (Read1)
91 bp (Read2)
Reference genome
GRCh38
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2
Data use policy
JGAP000013 Policy
NBDC data sharing policy (JGAP000001)

scTCR-seq

Materials and participants
6 healthy individuals (JGAD000957/Dataset 2)
70s-90s: 2
100s: 2
110s: 2
  • Health status
    Healthy
  • Subject count
    6 (Individual)
  • Age group
    Elderly
Sample description
RNAs extracted from peripheral blood T cells stimulated for 6 hours with PMA and ionomycin
  • Tissue
    Peripheral blood, T cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
TCR-seq
Target
N/A
Reagent kit
Chromium Single Cell V(D)J Enrichment Kit, Human T Cell
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
72 bp (Read1)
91 bp (Read2)
Reference genome
GRCh38
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2
Data use policy
JGAP000013 Policy
NBDC data sharing policy (JGAP000001)

scCITE-seq

Materials and participants
6 healthy individuals (JGAD000957/Dataset 2)
70s-90s: 2
100s: 2
110s: 2
  • Health status
    Healthy
  • Subject count
    6 (Individual)
  • Age group
    Elderly
Sample description
RNAs extracted from peripheral blood T cells stimulated for 6 hours with PMA and ionomycin
  • Tissue
    Peripheral blood, T cell
  • Tumor / normal
    Normal
Sample provider
N/A
Experimental method
CITE-seq
Target
CD3, CD4, CD8, CD25, CD27, CD28, CD45RA, CD45RO, CD95, TCRD, CCR7, IL7RA, PD-1, CD161, KLRG1, CD56
Reagent kit
Chromium Single Cell 5' Feature Barcode Library Kit
Fragmentation
Enzymatic fragmentation
Platform
MGI DNBSEQ-G400
Read type
Paired-end
Read length
0 bp (Read1)
15 bp (Read2)
Reference genome
N/A
Mapping
Cell Ranger 6.1.2
QC and filtering
Cell Ranger 6.1.2
Analysis method
Cell Ranger 6.1.2
Data use policy
JGAP000013 Policy
NBDC data sharing policy (JGAP000001)