NBDC Research ID: hum0379.v1
SUMMARY
Aims: Analysis of immune cells in autoimmune diseases
Methods: single-cell RNA+VDJ-seq
Participants/Materials: myasthenia gravis (MG) 2 cases, multiple sclerosis (MS) 4 cases, systemic lupus erythematosus (SLE) 3 cases, 3 healthy cases
Dataset ID | Type of Data | Criteria | Release Date |
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JGAS000578 | NGS (scRNA-seq, scVDJ-seq) | Controlled-access (Type I) | 2023/11/20 |
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MOLECULAR DATA
Participants/Materials |
MG (ICD10: G70.0): 2 cases (CD4+ T cell and B cell specimens each) MS (ICD10: G35): 4 cases (3 cases of CD4+ T cell and B cell specimens each + 1 mixed CD4+ T cell and B cell specimen) SLE (ICD10: M32.9): 3 cases (3 cases of CD4+ T cell and B cell specimens each + 1 CD4+ T cell pecimen) 3 healthy cases (CD4+ T cell and B cell specimens each) |
Targets | scRNA-seq and scVDJ-seq |
Target Loci for Capture Methods | - |
Platform | Illumina [NovaSeq 6000] |
Library Source | RNAs extracted from CD4+ T cell and B cell isolated from peripheral blood |
Cell Lines | - |
Library Construction (kit name) |
scRNA-seq: Chromium Next GEM Single Cell 5’ Kit v2 scVDJ-seq: Chromium Single Cell Human BCR Amplification Kit or Chromium Single Cell Human TCR Amplification Kit |
Fragmentation Methods | included in the above library construction kit |
Spot Type | Paired-end |
Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 91 bp |
Japanese Genotype-phenotype Archive Dataset ID | JGAD000704 |
Total Data Volume | 511 GB (fastq) |
Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Shimon Sakaguchi
Affiliation: Experimental Immunology, IFReC, Osaka University
Project / Group Name: -
Name | Title | Project Number |
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KAKENHI Grant-in-Aid for Specially Promoted Research | Study of the function and development of regulatory T cells | 16H06295 |
Leading Advanced Projects for medical innovation (LEAP), Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED) | Study of Immunoregulatory technology targeting regulatory T cells | JP18gm0010005 |
PUBLICATIONS
Title | DOI | Dataset ID | |
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1 | |||
2 |
USRES (Controlled-access Data)
Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
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