NBDC Research ID: hum0284.v1
SUMMARY
Aims: To clarify expressional changes from precancerous to malignancy by single-cell RNA-seq analysis of breast cancer clinical specimens
Methods: Cells were isolated from the surgically resected tumor tissue. Single-cell sequence library was constructed using 10xChromium kit. Single-cell gene expression analysis was performed by a next-generation sequencer.
Participants/Materials: Primary tumor samples collected from breast cancer patients
Dataset ID | Type of Data | Criteria | Release Date |
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JGAS000309 | NGS (scRNA-seq) | Controlled-access (Type I) | 2023/01/19 |
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MOLECULAR DATA
Participants/Materials |
breast invasive carcinoma (ICD10: C50): 4 cases surgically resected tumor tissue: 4 samples |
Targets | scRNA-seq |
Target Loci for Capture Methods | - |
Platform | Illumina [NovaSeq 6000] |
Library Source | RNAs extracted from isolated cells abteined from surgically resected tumor tissues. |
Cell Lines | - |
Library Construction (kit name) | Chromium Next GEM Single Cell 3' Reagent Kits v3.1 |
Fragmentation Methods | Chromium Next GEM Single Cell 3' Reagent Kits v3.1 |
Spot Type | Single-end |
Read Length (without Barcodes, Adaptors, Primers, and Linkers) | - |
Japanese Genotype-phenotype Archive Dataset ID | JGAD000420 |
Total Data Volume | 134.5 GB (fastq) |
Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Jun Nakayama
Affiliation: Division of Cellular Signaling, National Cancer Center Research Institute
Project / Group Name: Laboratory of Integrative Oncology
Funds / Grants (Research Project Number):
Name | Title | Project Number |
---|---|---|
KAKENHI Grant-in-Aid for JSPS Research Fellows | Analysis of Cancer Evolution by Single Cell Amplicomics | 20J01794 |
PUBLICATIONS
Title | DOI | Dataset ID | |
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1 | Single-Cell Transcriptome Profiling Reveals Intratumoral Heterogeneity and Molecular Features of Ductal Carcinoma In Situ | doi: 10.1158/0008-5472.CAN-22-0090 | JGAD000420 |
2 |
USRES (Controlled-access Data)
Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
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