NBDC Research ID: hum0284.v1
SUMMARY
Aims: To clarify expressional changes from precancerous to malignancy by single-cell RNA-seq analysis of breast cancer clinical specimens
Methods: Cells were isolated from the surgically resected tumor tissue. Single-cell sequence library was constructed using 10xChromium kit. Single-cell gene expression analysis was performed by a next-generation sequencer.
Participants/Materials: Primary tumor samples collected from breast cancer patients
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000309 | NGS (scRNA-seq) | Controlled-access (Type I) | 2023/01/19 |
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MOLECULAR DATA
| Participants/Materials |
breast invasive carcinoma (ICD10: C50): 4 cases surgically resected tumor tissue: 4 samples |
| Targets | scRNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | RNAs extracted from isolated cells abteined from surgically resected tumor tissues. |
| Cell Lines | - |
| Library Construction (kit name) | Chromium Next GEM Single Cell 3' Reagent Kits v3.1 |
| Fragmentation Methods | Chromium Next GEM Single Cell 3' Reagent Kits v3.1 |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | - |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000420 |
| Total Data Volume | 134.5 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Jun Nakayama
Affiliation: Division of Cellular Signaling, National Cancer Center Research Institute
Project / Group Name: Laboratory of Integrative Oncology
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for JSPS Research Fellows | Analysis of Cancer Evolution by Single Cell Amplicomics | 20J01794 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Single-Cell Transcriptome Profiling Reveals Intratumoral Heterogeneity and Molecular Features of Ductal Carcinoma In Situ | doi: 10.1158/0008-5472.CAN-22-0090 | JGAD000420 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
