NBDC Research ID: hum0247.v1

 

SUMMARY

Aims: Establishment of a tumor infiltrating lymphocytes (TIL) therapy for malignant melanoma

Methods: Exome sequencing of melanoma tissues for TIL production and paired peripheral blood mononuclear cells (PBMC)

Participants/Materials: human melanoma and paired PBMC

 

Dataset IDType of DataCriteriaRelease Date
JGAS000249 NGS (Exome) Controlled-access (Type I) 2021/01/04
DRA010773 NGS (Exome) Unrestricted-access 2021/01/04

*Release Note

*Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more

*When the research results including the data which were downloaded from NHA/DRA, are published or presented somewhere, the data user must refer the papers which are related to the data, or include in the acknowledgment. Learn more

 

MOLECULAR DATA

JGAS000249 / DRA010773

Participants/Materials

melanoma (ICD10: C43.3, C43.7): 3 cases

    tumor tissue: 3 samples

    non-tumor tissue (PBMC): 3 samples

Targets Exome
Target Loci for Capture Methods -
Platform Illumina [HiSeq 1500]
Library Source DNAs extracted from tumor tissues and PBMCs of melanoma patients
Cell Lines -
Library Construction (kit name) SureSelect Human All Exon Kit V6
Fragmentation Methods Ultrasonic fragmentation (Covaris)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 100 bp
Japanese Genotype-phenotype Archive Dataset ID / DDBJ Sequence Read Archive ID

non-tumor tissues: JGAD000349

tumor tissues: DRA010773

Total Data Volume

non-tumor tissues: 25 GB (fastq)

tumor tissues: 15 GB (bam [ref: hg19])

Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Takashi Iwata

Affiliation: Department of Obsterics and Gynecology, Keio University School of Medicine

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
Practical Research for Innovative Cancer Control, Japan Agency for Medical Research and Development (AMED) Pilot study of adoptive cell therapy using cultured tumor infiltrating lymphocytes with lympho-depleting non-myeloablative preconditioning for patients with metastatic melanoma JP17ck0106158

 

PUBLICATIONS

TitleDOIDataset ID
1 Pilot study of adoptive cell therapy using cultured tumor infiltrating lymphocytes for Japanese melanoma patients refractory to immune-checkpoint inhibitors doi: 10.1111/cas.15009

JGAD000349

DRA010773

2

 

USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use