NBDC Research ID: hum0223.v1

 

SUMMARY

Aims: To clarify of the disease status of myeloma and lymphoma by somatic gene mutation analyses

Methods: gene mutaion analyses using NGS technology at the point of diagnosis, relapse, or proceeding disease

Participants/Materials: multiple myeloma

 

Dataset IDType of DataCriteriaRelease Date
JGAS000232 NGS (Target Capture) Controlled-access (Type I) 2020/09/01

*Release Note

* Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more

 

MOLECULAR DATA

JGAS000232

Participants/Materials

multiple myeloma (ICD10: C90.0): 7 cases

    myeloma cells from bone marrow aspirates: total 30 samples

Targets Target Capture
Target Loci for Capture Methods 126 genes
Platform Thermo Fisher Scientific [Ion Proton]
Library Source DNAs extracted from myeloma cells isolated from bone marrow samples at the point of diagnosis, relapse, or proceeding disease
Cell Lines -
Library Construction (kit name) Ion Ampliseq Designer™
Fragmentation Methods -
Spot Type Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 3-1754 bp
QC/Filtering Methods TaqMan® Assay
Deduplication -
Mapping Methods TMAP
Reference Genome Sequence hg19
Coverage (Depth) 2815
Detecting Methods for Variation) Variant Caller
Japanese Genotype-phenotype Archive Dataset ID JGAD000326
Total Data Volume 87 GB(vcf)
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Yasuhito Terui

Affiliation: Dept of Hematology Oncology, The Cancer Institute Hospital of JFCR

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
-

 

PUBLICATIONS

TitleDOIDataset ID
1 The clonal evolution during long-term clinical course of multiple myeloma doi: 10.1007/s12185-020-02979-7 JGAD000326
2

 

USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use