NBDC Research ID: hum0173.v1

 

SUMMARY

Aims: to explore the effects of genetic and environmental factors, particularly the fetal environment and post-birth living environment, on children's health and to identify potential biomarkers for these effects.

Methods: Reduced Representation Bisulfite Sequencing (RRBS)

Participants/Materials: human umbilical cords

 

Dataset IDType of DataCriteriaRelease Date
JGAS000171 NGS (RRBS) Controlled-access (Type I) 2019/04/23

*Release Note

* Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data. Learn more

 

MOLECULAR DATA

JGAS000171

Participants/Materials: human umbilical cords: 30 samples
Targets RRBS
Target Loci for Capture Methods -
Platform Illumina [NextSeq]
Library Source DNA extracted from human umbilical cords
Cell Lines -
Library Construction (kit name) RRBS method (Ovation RRBS Methyl-Seq System 1-16, EpiTect Fast Bisulfite Conversion kits)
Fragmentation Methods MspI restriction enzymes for target DNA digestion
Spot Type Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 75 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000249
Total Data Volume 44 GB (bam [ref: GRCh38])
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Chisato Mori

Affiliation: Chiba University・Center for Preventive Medical Sciences

Project / Group Name: Chiba Study of Mother and Child Health (C-MACH)

URL: -

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research (A) Establishment of new preventive medicine focusing on fetal period with children's health effect evaluation method by using multi-omics analysis 16H01781

 

PUBLICATIONS

TitleDOIDataset ID
1 Chiba study of Mother and Children's Health (C-MACH): cohort study with omics analyses. doi: 10.1136/bmjopen-2015-010531 JGAD000249
2 DNA methylome of human neonatal umbilical cord: Enrichment of differentially methylated regions compared to umbilical cord blood DNA at transcription factor genes involved in body patterning and effects of maternal folate deficiency or children’s sex. doi: 10.1371/journal.pone.0214307 JGAD000249

 

USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use