NBDC Research ID: hum0173.v1
SUMMARY
Aims: to explore the effects of genetic and environmental factors, particularly the fetal environment and post-birth living environment, on children's health and to identify potential biomarkers for these effects.
Methods: Reduced Representation Bisulfite Sequencing (RRBS)
Participants/Materials: human umbilical cords
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000171 | NGS (RRBS) | Controlled-access (Type I) | 2019/04/23 |
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MOLECULAR DATA
| Participants/Materials: | human umbilical cords: 30 samples |
| Targets | RRBS |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NextSeq] |
| Library Source | DNA extracted from human umbilical cords |
| Cell Lines | - |
| Library Construction (kit name) | RRBS method (Ovation RRBS Methyl-Seq System 1-16, EpiTect Fast Bisulfite Conversion kits) |
| Fragmentation Methods | MspI restriction enzymes for target DNA digestion |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 75 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000249 |
| Total Data Volume | 44 GB (bam [ref: GRCh38]) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Chisato Mori
Affiliation: Chiba University・Center for Preventive Medical Sciences
Project / Group Name： Chiba Study of Mother and Child Health (C-MACH)
URL： -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (A) | Establishment of new preventive medicine focusing on fetal period with children's health effect evaluation method by using multi-omics analysis | 16H01781 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Chiba study of Mother and Children's Health (C-MACH): cohort study with omics analyses. | doi: 10.1136/bmjopen-2015-010531 | JGAD000249 |
| 2 | DNA methylome of human neonatal umbilical cord: Enrichment of differentially methylated regions compared to umbilical cord blood DNA at transcription factor genes involved in body patterning and effects of maternal folate deficiency or children’s sex. | doi: 10.1371/journal.pone.0214307 | JGAD000249 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
