NBDC Research ID: hum0168.v1

 

SUMMARY

Aims: Analysis of genetic background of adenomyosis

Methods: Whole exome sequencing was performed on fresh fronzen samples from adenomyosis individuals

Participants/Materials: 51 patients of adenomyosis

 

Dataset IDType of DataCriteriaRelease Date
JGAS000169 NGS (Exome) Controlled-access (Type I) 2019/11/08

*Release Note

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MOLECULAR DATA

JGAS000169

Participants/Materials adenomyosis: 51 cases
Targets Exome
Target Loci for Capture Methods -
Platform Illumina [HiSeq 2500]
Library Source DNAs extracted from fresh frozen samples (adenomyosis, co-occurring endometriosis, co-occurring leiomyoma, co-occurring ovarian cancer, normal myometrium, monocytic cells in ascitic fluid, peripheral blood cells) from adenomyosis individuals
Cell Lines -
Library Construction (kit name) NEBNext Ultra DNA library prep kit for Illumina, Agilent V6 plus custom probes
Fragmentation Methods Ultrasonic fragmentation (Covaris)
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 125 bp
Japanese Genotype-phenotype Archive Dataset ID JGAD000247
Total Data Volume 2 TB (bam [ref: hg38])
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Masahito Kawazu

Affiliation: National Cancer Center Research Institute

Project / Group Name: Division of Cellular Signaling

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research (C) Establishment of highly sensitive SNV detection method of WES for uterine adenomyosis by LCM method. 19K07708

 

PUBLICATIONS

TitleDOIDataset ID
1 Uterine adenomyosis is an oligoclonal disorder associated with KRAS mutations doi: 10.1038/s41467-019-13708-y JGAD000247
2

 

USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use