NBDC Research ID: hum0168.v1
SUMMARY
Aims: Analysis of genetic background of adenomyosis
Methods: Whole exome sequencing was performed on fresh fronzen samples from adenomyosis individuals
Participants/Materials: 51 patients of adenomyosis
Dataset ID | Type of Data | Criteria | Release Date |
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JGAS000169 | NGS (Exome) | Controlled-access (Type I) | 2019/11/08 |
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MOLECULAR DATA
Participants/Materials | adenomyosis: 51 cases |
Targets | Exome |
Target Loci for Capture Methods | - |
Platform | Illumina [HiSeq 2500] |
Library Source | DNAs extracted from fresh frozen samples (adenomyosis, co-occurring endometriosis, co-occurring leiomyoma, co-occurring ovarian cancer, normal myometrium, monocytic cells in ascitic fluid, peripheral blood cells) from adenomyosis individuals |
Cell Lines | - |
Library Construction (kit name) | NEBNext Ultra DNA library prep kit for Illumina, Agilent V6 plus custom probes |
Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
Spot Type | Paired-end |
Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 125 bp |
Japanese Genotype-phenotype Archive Dataset ID | JGAD000247 |
Total Data Volume | 2 TB (bam [ref: hg38]) |
Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Masahito Kawazu
Affiliation: National Cancer Center Research Institute
Project / Group Name: Division of Cellular Signaling
Funds / Grants (Research Project Number):
Name | Title | Project Number |
---|---|---|
KAKENHI Grant-in-Aid for Scientific Research (C) | Establishment of highly sensitive SNV detection method of WES for uterine adenomyosis by LCM method. | 19K07708 |
PUBLICATIONS
Title | DOI | Dataset ID | |
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1 | Uterine adenomyosis is an oligoclonal disorder associated with KRAS mutations | doi: 10.1038/s41467-019-13708-y | JGAD000247 |
2 |
USRES (Controlled-access Data)
Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
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