NBDC Research ID: hum0519.v1
SUMMARY
Aims: In patients with autoimmune diseases, it is known that immune cells react against components of their own body. By identifying and characterizing immune cells that respond to self-antigens - components that should normally be absent or rendered immunologically inert - we aim to elucidate the mechanisms underlying autoimmune pathology and contribute to the advancement of medical science.
Methods: Single cell RNA sequencing/TCR repertoire analyses
Participants/Materials: Labial salivary gland tissue obtained from patients with Sjögren's syndrome who tested positive for anti-SSA antibodies
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000808 | NGS (scRNA-seq/TCR repertoire) | Controlled-access (Type I) | 2026/02/10 |
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MOLECULAR DATA
| Participants/Materials |
Sjögren's syndrome (ICD10: M350): 8 cases labial salivary glands: 8 samples |
| Targets | scRNA-seq/TCR repertoire |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq X] |
| Library Source | RNAs extracted from T cell obtained from labial salivary glands |
| Cell Lines | - |
| Library Construction (kit name) | Chromium Next GEM Single Cell 5' Library |
| Fragmentation Methods | included in the above library construction kit |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 150 bp |
| Mapping Methods | Cell Ranger (version 5.0.1) |
| Reference Genome Sequence | GRCh38 (reference_accession: GCA_000001405.15) |
| Detecting method for read count (software) | Cell Ranger (version 5.0.1) |
| Gene number | Approximately 1000-1500 |
| Repertoire analysis Methods (software) | Cell Ranger (version 5.0.1) |
| QC | Cell Ranger (version 5.0.1) |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000950 |
| Total Data Volume | 270.2 GB (bam, csv) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Masaru Takeshita
Affiliation: Division of Rheumatology, Keio University
Project / Group Name: -
| Name | Title | Project Number |
|---|---|---|
| JSR・JKiC Next-generation Project | Establishing Disease Control Methods Through Detailed Analysis of Antigen Specificity in Autoimmune Diseases | - |
| KAKENHI Grant-in-Aid for Early-Career Scientists | Single cell integrated analysis of antigen specificity and gene expression for the cure of autoimmune diseases | 20K17430 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | Elucidation of the Mechanisms of Autoantibody Production at Lesion Sites in Autoimmune Diseases | 22K08528 |
| Keio University Academic Promotion Fund | Elucidation of Autoimmune Mechanisms at Lesion Sites in Autoimmune Diseases and Development of Antigen-Specific Immunosuppressive Therapies | - |
| Keio University Fukuzawa Memorial Fund | Comprehensive Understanding of Autoimmunity and Practical Application of Antigen-Specific Immunosuppressive Therapies | - |
| Keio University Next-Generation Research Promotion Program | Comprehensive Analysis of Salivary Gland Lesions as Sites of Autoimmune Responses | - |
| Mochida Memorial Foundation for Medical and Pharmaceutical Research — Research Grant — | lucidation of Autoimmune Mechanisms in Lesional Tissues of Sjögren’s Syndrome and Development of Antigen-Specific Immunosuppressive Therapies | - |
| Kowa Life Science Foundation — Research Grant — | Elucidation of Molecular Mechanisms Involved in Autoimmune Responses at Lesion Sites in Sjögren’s Syndrome | - |
| Takeda Science Foundation — Medical Research Grant — | Elucidation of Autoimmune Mechanisms at Lesion Sites in Autoimmune Diseases | - |
| The Astellas Foundation for Research on Metabolic Disorders — Research Grant — | Identification of molecular targets involved in autoimmune responses in autoimmune diseases | - |
| The SENSHIN Medical Research Foundation — Research Grant — | Development of autoantigen-specific immunosuppressive therapy for Sjögren's Disease | - |
| The Nakatomi Foundation — Research Grant — | Creation of Artificial Synovium Using Synovial Fibroblasts Derived from Rheumatoid Arthritis | - |
| The Takahashi Industrial Economic Research Foundation — Research Grant — | Development of Autoantigen-Specific Immunosuppressive Therapy for autoimmune disease | - |
| The Waksman Foundation of Japan INC — Research Grant — | Molecular Pathogenesis Analysis of Autoimmune Diseases from Both Autoimmunity and Inflammation Perspectives | - |
| Okinaka Memorial Institute for Medical Research | Elucidation of the molecular mechanisms involved in maintaining autoimmunity | - |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | |||
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
