NBDC Research ID: hum0465.v1
SUMMARY
Aims: Among acute leukemias, mixed phenotype acute leukemias (mixed phenotype acute leukemia: MAPL), which have both acute myeloid and acute lymphocytic leukemia traits, are generally considered resistant to chemotherapy, especially those with t(9;22) MPAL has a poorer prognosis than other forms of MPAL and is a challenge to overcome. MPAL accounts for less than 1% of all acute leukemias, and the rarity of this disease makes it difficult to conduct clinical studies to develop treatments for the disease. Therefore, we planned to establish cell lines based on acute leukemia cells (JMPAL-1) obtained from an MPAL patient and to analyze information such as gene mutations related to disease development based on these cell lines for therapeutic purposes.
Methods: A cell line (JMPAL-1) was established from the acute leukemia cells of an MPAL patient. Genomic analysis of JMPAL-1 was performed.
Participants/Materials: mixed phenotype acute leukemia
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000721 | NGS (Exome, RNA-seq) | Controlled-access (Type I) | 2024/07/25 |
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MOLECULAR DATA
| Participants/Materials |
mixed phenotype acute leukemia (ICD10: C950): 1 case JMPAL-1: 1 sample |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NextSeq 500] |
| Library Source | DNA extracted from cell line |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect XT Human All Exon V7 kit |
| Fragmentation Methods | Ultrasonic fragmentation |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 200 bp |
| Mapping Methods | Genomon2 |
| Mapping Quality | mapped bases/mismapped bases 10,837,871,473 / 32,768,322 |
| Reference Genome Sequence | hg19 |
| Coverage (Depth) | 30x ratio = 0.945 |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000854 |
| Total Data Volume | 10 GB (bam) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
mixed phenotype acute leukemia (ICD10: C950): 1 case JMPAL-1: 1 sample |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NextSeq 500] |
| Library Source | RNA extracted from cell line |
| Cell Lines | - |
| Library Construction (kit name) | TruSeq RNA Library Prep Kit |
| Fragmentation Methods | TruSeq RNA Library Prep Kit |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 150 bp |
| Mapping Methods | Genomon-RNA |
| Mapping Quality | - |
| Reference Genome Sequence | hg19 |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000854 |
| Total Data Volume | 10 GB (bam) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Hiroto Ishii
Affiliation: Division of Clinical Oncology & Hematology, Department of Internal Medicine, The Jikei University School of Medicine
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | |||
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
