NBDC Research ID: hum0456.v1
SUMMARY
Aims: Comprehensive analysis of genetic and clinical information on parasellar base skull tumors and multiple malignant gliomas is expected to elucidate the pathogenesis of the diseases and provide clinically useful results leading to prevention and the development of personalized medicine.
Methods: The cell suspension from the resected tumor specimens was processed according to 10×Genomics specifications, using the 10×Chromium Next GEM single-cell 3ʹ reagent kit and library construction kit. After quantification and quality assessment of the library, single-cell RNA sequencing was performed on the NovaSeq 6000.
Participants/Materials: Surgical resection specimens from 10 craniopharyngiomas
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000722 | NGS (scRNA-seq) | Controlled-access (Type I) | 2024/07/17 |
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MOLECULAR DATA
| Participants/Materials |
craniopharyngioma (ICD10: C752): 10 cases tumor tissues: 10 samples |
| Targets | scRNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | RNAs extracted from tumor tissues |
| Cell Lines | - |
| Library Construction (kit name) | Chromium Next GEM Single Cell 3ʹ Reagent Kits |
| Fragmentation Methods | Enzymatic fragmentation |
| Spot Type | |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000855 |
| Total Data Volume | 296.3 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Tatsuma Matsuda
Affiliation: Department of Molecular Diagnosis, Graduate School of Medicine Chiba University
Project / Group Name: Molecular Pathological exprolation of Tumor Formation and Functional Differentiation Using Parasellar Skull Base Tumors and Malignant Glioma Tissues
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (B) | Fundamental research on age-related long non-coding RNAs in functional alterations associated with adipose and vascular tissue ageing | 21H02974 |
| KAKENHI Grant-in-Aid for Scientific Research (B) | Molecular pathological research on obesity and diabetes based on analysis of mitochondrial complexes and regulatory mechanisms | 19H03708 |
| KAKENHI Grant-in-Aid for Scientific Research (B) | Regulation of chromatin function and iPS reprogramming by the p53 transcription factor complex | 22300325 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Deciphering craniopharyngioma subtypes: Single-cell analysis of tumor microenvironment and immune networks | JGAD000855 | |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
