NBDC Research ID: hum0423.v1

SUMMARY

Aims: To elucidate the pathomechanism of inflammatory muscle diseases using multi-omics analysis

Methods: Short read and long read RNA sequencing

Participants/Materials: 22 sarcoid myopathy patients

URL： https://mgc.ncnp.go.jp/index_en.php

Dataset ID	Type of Data	Criteria	Release Date
JGAS000636	NGS (RNA-seq)	Controlled-access (Type I)	2024/10/23

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MOLECULAR DATA


Participants/Materials	sarcoid myopathy (ICD10: D86.8, G73.7): 22 cases (22 samples) Short read RNA sequencing: 16 cases (16 samples) Long read RNA sequencing: 6 cases (6 samples)
Targets	RNA-seq
Target Loci for Capture Methods	-
Platform	MGI [DNBSEQ] Nanopore [PromethION]
Library Source	RNAs extracted from muscular lesions
Cell Lines	-
Library Construction (kit name)	MGI: Not available Nanopore: cDNA-PCR Sequencing Kit (SQK-PCS111)
Fragmentation Methods	MGI: Reverse transcription by random priming Nanopore: -
Spot Type	MGI: Paired-end Nanopore: Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	MGI: 100 bp Nanopore: 918 bp on average
Japanese Genotype-phenotype Archive Dataset ID	JGAD000766
Total Data Volume	523.3 GB (fastq)
Comments (Policies)	NBDC policy

Principal Investigator: Aritoshi Iida

Affiliation: Medical Genome Center, National Center of Neurology and Psychiatry

Project / Group Name: Section of Clinical Genome Analysis, Department of Clinical Genome Analysis

URL: https://mgc.ncnp.go.jp/index_en.php

Funds / Grants (Research Project Number):

Name	Title	Project Number
Practical Research Project for Rare/Intractable Diseases, Japan Agency for Medical Research and Development (AMED)	Comprehensive elucidation of the pathomechanism of muscle diseases by multi-omics analyses	JP22ek0109490

	Title	DOI	Dataset ID
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Principal Investigator	Affiliation	Country/Region	Research Title	Data in Use (Dataset ID)	Period of Data Use