NBDC Research ID: hum0367.v1
SUMMARY
Aims: Hematopoietic malignancies such as leukemia develop as a result of the accumulation of structural and functional abnormalities in the genome and epigenome in the originating undifferentiated hematopoietic stem and progenitor cells. However, the details of the pathogenesis and the processes leading to the onset of these disorders remain unresolved, and fundamental treatments based on the pathogenic mechanisms have not been established. In this study, we aim to clarify the genomic and epigenomic abnormalities involved in the pathogenesis of hematological diseases using comprehensive genomic analysis and to search for key molecules that will lead to accurate diagnosis and drug discovery for hematological diseases based on the mechanisms.
Methods: DNAs extracted from bone marrow (BM) and lymph node (LN) samples at the time of diagnosis were subjected to a target capture sequencing analysis by using the Human Myeloid Neoplasms QIAseq Targeted DNA Panel.
Participants/Materials: Langerhans cell histiocytosis (LCH)
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000558 | NGS (Target Capture) | Controlled-access (Type I) | 2021/09/21 |
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| Participants/Materials |
LCH (ICD10: C96.6): 1 case BM and LN: 1 sample each |
| Targets | Target Capture |
| Target Loci for Capture Methods | Human Myeloid Neoplasms QIAseq Targeted DNA Panel |
| Platform | Illumina [MiSeq] |
| Library Source | DNAs extracted from BM and LN |
| Cell Lines | - |
| Library Construction (kit name) | PCR amplicon sequencing (Human Myeloid Neoplasms QIAseq Targeted DNA Panel) |
| Fragmentation Methods | Enzymatic fragmentation |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 250 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000680 |
| Total Data Volume | 736.7 MB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Arinobu Tojo
Affiliation: Department of Hematology/Oncology, Institute of Medical Science, University of Tokyo
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Case report: Common clonal origin of concurrent langerhans cell histiocytosis and acute myeloid leukemia | doi: 10.3389/fonc.2022.974307 | JGAD000680 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
