NBDC Research ID: hum0352.v1
SUMMARY
Aims: To elucidate the functions of DNA repair proteins and RNA expression that are involved in the therapeutic efficacy and adverse events of radiotherapy for lung cancer. In particular, this is an exploratory study to identify proteins and RNAs involved in the expression of proteins that are likely to be involved in the prediction of radiotherapy response and adverse events.
Methods: Peripheral blood samples were collected from the patients with lung cancer who gave consent for this study. RNA-sequencing analysis was performed with the RNAs extracted from the blood samples.
Participants/Materials: Patients who have undergone radiotherapy for primary lung cancer at our hospital between 2019 and 2020 will be considered. Among them, we will include cases in which it is possible to determine the effect of treatment and evaluate adverse events before and, and whose blood can be drawn at 3 points: before radiotherapy, at the end of treatment, and 2-4 weeks after treatment.
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000545 | NGS (RNA-seq) | Controlled-access (Type I) | 2022/09/20 |
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MOLECULAR DATA
| Participants/Materials |
primary lung cancer (ICD10: C340): 5 cases blood obtained before radiotherapy, at the end of treatment, and 2-4 weeks after treatment, 15 samples in total |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | RNAs extracted from peripheral blood cells |
| Cell Lines | - |
| Library Construction (kit name) | TruSeq Stranded mRNA LT Sample Prep Kit |
| Fragmentation Methods | TruSeq Stranded mRNA LT Sample Prep Kit |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000664 |
| Total Data Volume | 189.0 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Masanori Someya
Affiliation: Department of Radiology, Sapporo Medical University, School of Medicine
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (C) | Practical application of individualized radiotherapy using liquid biopsy | 21K07680 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Combined chemoradiotherapy and programmed cell death-ligand 1 blockade leads to changes in the circulating T-cell receptor repertoire of patients with non-small-cell lung cancer | doi: 10.1111/cas.15566 | JGAD000664 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
