NBDC Research ID: hum0309.v1

SUMMARY

Aims: Evaluation of properties between sebum and stratum corneum RNA

Methods: AmpliSeq Transcriptome analysis was performed to analyze human mRNA expressions in sebum or stratum corneum RNA

Participants/Materials: ten males (facial sebum 10 samples and facial stratum corneum 10 samples)

Dataset ID	Type of Data	Criteria	Release Date
JGAS000418	NGS (RNA-seq)	Controlled-access (Type I)	2022/03/11

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MOLECULAR DATA


Participants/Materials	10 healthy males facial sebum 10 samples and facial stratum corneum 10 samples
Targets	RNA-seq
Target Loci for Capture Methods	-
Platform	Thermo Fisher Scientific [Ion S5]
Library Source	RNAs extracted from sebum and stratum corneum
Cell Lines	-
Library Construction (kit name)	Ion AmpliSeq Transcriptome Human Gene Expression Kit
Fragmentation Methods	-
Spot Type	Single-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	110 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000535
Total Data Volume	67.4 GB (bam)
Comments (Policies)	NBDC policy

Principal Investigator: Takayoshi Inoue

Affiliation: Biological Science Laboratories, Kao Corporation

Project / Group Name: Sebum RNA project

Funds / Grants (Research Project Number):

Name	Title	Project Number
-	-	-

	Title	DOI	Dataset ID
1	Non-invasive human skin transcriptome analysis using mRNA in skin surface lipids	doi: 10.1038/s42003-022-03154-w	JGAD000535
2

Principal Investigator	Affiliation	Country/Region	Research Title	Data in Use (Dataset ID)	Period of Data Use
Michiaki Hamada	Faculty of Science and Engineering, Waseda University	Japan	Construction of RNA-targeted Drug Discovery Database	JGAD000535	2022/12/26-2025/03/31