NBDC Research ID: hum0304.v1
SUMMARY
Aims: Taxanes are key drugs in the treatment of breast cancer, but some breast cancers are resistant to taxanes. We used whole exome-sequencing (WES) technology to investigate mechanisms of taxane resistance in human breast cancer.
Methods: DNAs extracted from tumor tissues and peripheral blood cells of patients with breast cancer whose tumors responded well to anthracycline treatment but grew rapidly during neoadjuvant taxane-based chemotherapy were used for WES analysis and somatic mutations in the candidate genes were identified.
Participants/Materials: Six patients with breast cancer whose tumors grew rapidly during neoadjuvant taxane-based chemotherapy
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000370 | NGS (Exome) | Controlled-access (Type I) | 2022/04/08 |
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MOLECULAR DATA
| Participants/Materials |
breast cancer (ICD10: C50): 6 cases tumor tissue: 6 samples peripheral blood cells: 3 samples |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform |
tumor tissue: Illumina [HiSeq 2000] peripheral blood: Illumina [HiSeq 2500] |
| Library Source | DNAs extracted from tumor tissue and peripheral blood cells |
| Cell Lines | - |
| Library Construction (kit name) | Ultrasonic fragmentation |
| Fragmentation Methods | SureSelect Human ALL Exon V5 Kit |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 200 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000484 |
| Total Data Volume | 41.5 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Yumi Wanifuchi-Endo
Affiliation: Department of Breast Surgery, Graduate School of Medical Sciences, Nagoya City University
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (C) | Exome sequencing analysis in human breast cancer tissues showing resistance for taxanes. | 16K10469 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Discovering novel mechanisms of taxane resistance in human breast cancer by whole-exome sequencing | doi: 10.3892/ol.2021.13178 | JGAD000484 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
