NBDC Research ID: hum0287.v1

SUMMARY

Aims: Genome sequence comparison of human iPS cell lines

Methods: Genomic DNA was extracted from human iPS cell lines and whole genome sequence analysis was performed.

Participants/Materials: Human iPS cell lines

Dataset ID	Type of Data	Criteria	Release Date
JGAS000310	NGS (Target Capture)	Controlled-access (Type I)	2021/11/02

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Participants/Materials	Human iPS cell line: 5 lines
Targets	WGS
Target Loci for Capture Methods	-
Platform	Illumina [HiSeq X]
Library Source	gDNAs extracted from iPS cells
Cell Lines	-
Library Construction (kit name)	TruSeq DNA PCR Free (350)
Fragmentation Methods	Ultrasonic fragmentation (Covaris)
Spot Type	Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers)	150 bp
Japanese Genotype-phenotype Archive Dataset ID	JGAD000421
Total Data Volume	343 GB (fastq)
Comments (Policies)	NBDC policy

DATA PROVIDER

Principal Investigator: Shihori Yokobayashi

Affiliation: Center for iPS Cell Research and Application, Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University

Project / Group Name: -

Funds / Grants (Research Project Number):

Name	Title	Project Number
KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)	Chromatin dynamics linked to the epigenomic heterogeneity in human pluripotent stem cells	20H05387
KAKENHI Grant-in-Aid for Scientific Research (B)	Chromatin dynamics during primordial germ cell development in mammals	18H02613

	Title	DOI	Dataset ID
1	Inherent genomic properties underlie the epigenomic heterogeneity of human induced pluripotent stem cells	doi: 10.1016/j.celrep.2021.109909	JGAD000421
2

Principal Investigator	Affiliation	Country/Region	Research Title	Data in Use (Dataset ID)	Period of Data Use