NBDC Research ID: hum0280.v1
SUMMARY
Aims: To evaluate the tumor immune microenvironment (TIME) of lung cancer to develop a new immune based therapy.
Methods: We carried out RNA sequencing (RNA-seq) on 113 tumor tissues from patients with lung cancer. To evaluate the tumor immune microenviroment (TIME), we calculated the score (T-score; Glycolysis, Mitotic spindle, I-score; T cells, B cells, MHC-complex, S-score; angiogenesis, hypoxia, epithelial mesenchymal transition, cytokine production suppression) using single sample Gene Set Enrichment Analysis (ssGSEA).
Participants/Materials: We enrolled a total of 113 patients with lung cancer who underwent lung resection from August 2019 to June 2021 and obtained the fresh tumor samples.
Dataset ID | Type of Data | Criteria | Release Date |
---|---|---|---|
DRA012560 | NGS (RNA-seq) | Unrestricted-access | 2022/01/07 |
*When the research results including the data which were downloaded from NHA/DRA, are published or presented somewhere, the data user must refer the papers which are related to the data, or include in the acknowledgment. Learn more
MOLECULAR DATA
Participants/Materials |
lung cancer (ICD10: C34): 113 cases tumor tissue: 113 samples |
Targets | RNA-seq |
Target Loci for Capture Methods | - |
Platform | Illumina [NovaSeq 6000] |
Library Source | RNAs extracted from tumor tissues |
Cell Lines | - |
Library Construction (kit name) | SureSelect Human All Exon V6 |
Fragmentation Methods | Ultrasonic fragmentation |
Spot Type | Paired-end |
Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 150 bp |
DDBJ Sequence Read Archive ID | DRA012560 |
Total Data Volume | 2.5 TB (fastq) |
Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Hirokazu Matsushita
Affiliation: Division of Translational Oncoimmunology, Aichi Cancer Center Research Institute
Project / Group Name: Identification of target antigens and analysis of cancer microenvironments in lung cancer patients
Funds / Grants (Research Project Number):
Name | Title | Project Number |
---|---|---|
KAKENHI Grant-in-Aid for Scientific Research (C) | Investigation of Immunogenic cell death related to chemotherapy in circulating tumor cells | 20K09187 |
KAKENHI Grant-in-Aid for Scientific Research (B) | Specific immune responses against tumor antigens in long term survivors of cancer | 19H03528 |
Japanese Respiratory Foundation | The identification of CD4 positive cytotoxic T cell as a new target for immune-based therapy | - |
PUBLICATIONS
Title | DOI | Dataset ID | |
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1 | |||
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