NBDC Research ID: hum0280.v1
SUMMARY
Aims: To evaluate the tumor immune microenvironment (TIME) of lung cancer to develop a new immune based therapy.
Methods: We carried out RNA sequencing (RNA-seq) on 113 tumor tissues from patients with lung cancer. To evaluate the tumor immune microenviroment (TIME), we calculated the score (T-score; Glycolysis, Mitotic spindle, I-score; T cells, B cells, MHC-complex, S-score; angiogenesis, hypoxia, epithelial mesenchymal transition, cytokine production suppression) using single sample Gene Set Enrichment Analysis (ssGSEA).
Participants/Materials: We enrolled a total of 113 patients with lung cancer who underwent lung resection from August 2019 to June 2021 and obtained the fresh tumor samples.
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| DRA012560 | NGS (RNA-seq) | Unrestricted-access | 2022/01/07 |
*When the research results including the data which were downloaded from NHA/DRA, are published or presented somewhere, the data user must refer the papers which are related to the data, or include in the acknowledgment. Learn more
MOLECULAR DATA
| Participants/Materials |
lung cancer (ICD10: C34): 113 cases tumor tissue: 113 samples |
| Targets | RNA-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [NovaSeq 6000] |
| Library Source | RNAs extracted from tumor tissues |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon V6 |
| Fragmentation Methods | Ultrasonic fragmentation |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 150 bp |
| DDBJ Sequence Read Archive ID | DRA012560 |
| Total Data Volume | 2.5 TB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Hirokazu Matsushita
Affiliation: Division of Translational Oncoimmunology, Aichi Cancer Center Research Institute
Project / Group Name: Identification of target antigens and analysis of cancer microenvironments in lung cancer patients
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (C) | Investigation of Immunogenic cell death related to chemotherapy in circulating tumor cells | 20K09187 |
| KAKENHI Grant-in-Aid for Scientific Research (B) | Specific immune responses against tumor antigens in long term survivors of cancer | 19H03528 |
| Japanese Respiratory Foundation | The identification of CD4 positive cytotoxic T cell as a new target for immune-based therapy | - |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | |||
| 2 |
