NBDC Research ID: hum0280.v1

 

SUMMARY

Aims: To evaluate the tumor immune microenvironment (TIME) of lung cancer to develop a new immune based therapy.

Methods: We carried out RNA sequencing (RNA-seq) on 113 tumor tissues from patients with lung cancer. To evaluate the tumor immune microenviroment (TIME), we calculated the score (T-score; Glycolysis, Mitotic spindle, I-score; T cells, B cells, MHC-complex, S-score; angiogenesis, hypoxia, epithelial mesenchymal transition, cytokine production suppression) using single sample Gene Set Enrichment Analysis (ssGSEA).

Participants/Materials: We enrolled a total of 113 patients with lung cancer who underwent lung resection from August 2019 to June 2021 and obtained the fresh tumor samples.

 

Dataset IDType of DataCriteriaRelease Date
DRA012560 NGS (RNA-seq) Unrestricted-access 2022/01/07

*Release Note

*When the research results including the data which were downloaded from NHA/DRA, are published or presented somewhere, the data user must refer the papers which are related to the data, or include in the acknowledgment. Learn more

 

MOLECULAR DATA

DRA012560

Participants/Materials

lung cancer (ICD10: C34): 113 cases

    tumor tissue: 113 samples

Targets RNA-seq
Target Loci for Capture Methods -
Platform Illumina [NovaSeq 6000]
Library Source RNAs extracted from tumor tissues
Cell Lines -
Library Construction (kit name) SureSelect Human All Exon V6
Fragmentation Methods Ultrasonic fragmentation
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 150 bp
DDBJ Sequence Read Archive ID DRA012560
Total Data Volume 2.5 TB (fastq)
Comments (Policies) NBDC policy

 

DATA PROVIDER

Principal Investigator: Hirokazu Matsushita

Affiliation: Division of Translational Oncoimmunology, Aichi Cancer Center Research Institute

Project / Group Name: Identification of target antigens and analysis of cancer microenvironments in lung cancer patients

Funds / Grants (Research Project Number):

NameTitleProject Number
KAKENHI Grant-in-Aid for Scientific Research (C) Investigation of Immunogenic cell death related to chemotherapy in circulating tumor cells 20K09187
KAKENHI Grant-in-Aid for Scientific Research (B) Specific immune responses against tumor antigens in long term survivors of cancer 19H03528
Japanese Respiratory Foundation The identification of CD4 positive cytotoxic T cell as a new target for immune-based therapy -

 

PUBLICATIONS

TitleDOIDataset ID
1
2