NBDC Research ID: hum0254.v1



Aims: Investigation of the conditions for library preparation from cell-free DNAs (cfDNAs) using the newly developed adapter linkage reaction.

Methods: The cfDNA library prepared by the new technology was sequenced and analyzed for the properties of cfDNA.

Participants/Materials: cfDNA from healthy human plasma


Dataset IDType of DataCriteriaRelease Date
JGAS000257 NGS (WGS) Controlled-access (Type I) 2021/09/24

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Participants/Materials 5 healthy individuals (8 samples)
Targets WGS
Target Loci for Capture Methods -
Platform Illumina [Miseq, HiSeq X Ten]
Library Source cfDNA in plasma of peripheral blood
Cell Lines -
Library Construction (kit name) TACS-T4
Fragmentation Methods -
Spot Type Paired-end
Read Length (without Barcodes, Adaptors, Primers, and Linkers) 75-190 bp (119 bp on average)
Japanese Genotype-phenotype Archive Dataset ID JGAD000360
Total Data Volume 378.8 GB (fastq)
Comments (Policies) NBDC policy



Principal Investigator: Fumihito Miura

Affiliation: Department of Biochemistry, Kyushu University Graduate School of Medical Sciences

Project / Group Name: -

Funds / Grants (Research Project Number):

NameTitleProject Number
Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS), Japan Agency for Medical Research and Development (AMED) Advanced Methylomics: Support and Development JP20am0101103
KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Development and application of next-generation techniques for epigenome analysis 17H06305
Grant of The Clinical Research Promotion Foundation (2020) Comprehensive analysis of structure and modifications of plasma cell-free DNA -



TitleDOIDataset ID
1 Short single-stranded DNAs with putative non-canonical structures comprise a new class of plasma cell-free DNA. doi: 10.1186/s12915-021-01160-8 JGAD000360


USRES (Controlled-access Data)

Principal InvestigatorAffiliationCountry/RegionResearch TitleData in Use (Dataset ID)Period of Data Use