NBDC Research ID: hum0254.v1
SUMMARY
Aims: Investigation of the conditions for library preparation from cell-free DNAs (cfDNAs) using the newly developed adapter linkage reaction.
Methods: The cfDNA library prepared by the new technology was sequenced and analyzed for the properties of cfDNA.
Participants/Materials: cfDNA from healthy human plasma
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000257 | NGS (WGS) | Controlled-access (Type I) | 2021/09/24 |
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MOLECULAR DATA
| Participants/Materials | 5 healthy individuals (8 samples) |
| Targets | WGS |
| Target Loci for Capture Methods | - |
| Platform | Illumina [Miseq, HiSeq X Ten] |
| Library Source | cfDNA in plasma of peripheral blood |
| Cell Lines | - |
| Library Construction (kit name) | TACS-T4 |
| Fragmentation Methods | - |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 75-190 bp (119 bp on average) |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000360 |
| Total Data Volume | 378.8 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Fumihito Miura
Affiliation: Department of Biochemistry, Kyushu University Graduate School of Medical Sciences
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS), Japan Agency for Medical Research and Development (AMED) | Advanced Methylomics: Support and Development | JP20am0101103 |
| KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) | Development and application of next-generation techniques for epigenome analysis | 17H06305 |
| Grant of The Clinical Research Promotion Foundation (2020) | Comprehensive analysis of structure and modifications of plasma cell-free DNA | - |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Short single-stranded DNAs with putative non-canonical structures comprise a new class of plasma cell-free DNA. | doi: 10.1186/s12915-021-01160-8 | JGAD000360 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
