NBDC Research ID: hum0217.v1
SUMMARY
Aims: To clarify molecular and clinicopathological characteristics of colorectal serrated lesions
Methods: Targeted capture sequencing analysis of 39 genes mutated in colorectal cancers and/or serrated lesions
Participants/Materials: 50 serrated lesions, including TSAs, SSAs and microvesicular hyperplastic polyps
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000217 | NGS (Target Capture) | Controlled-access (Type I) | 2020/03/02 |
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MOLECULAR DATA
| Participants/Materials | colorectal serrated lesions (ICD10: C18): 50 cases |
| Targets | Target Capture |
| Target Loci for Capture Methods |
ACVR2A, AMER1, APC, ARID1A, ATM, AXIN2, BMP4, BRAF, CDKN2A, CHD7, CHD8, CTNNB1, DKK1, DKK2, DKK3, DKK4, FBXW7, FZD3, FZD10, GNAS, GSK3B, HRAS, KRAS, LRP5, MLH1, MSH6, NRAS, PIF1, PIK3CA, PTEN, RBL1, RNF43, SMAD4, SOX9, TCF7L2, TELO2, TGFBR2, TP53, XAF1 |
| Platform | Thermo Fisher Scientific [Ion Torrent] |
| Library Source | DNAs extracted from colorectal serrated lesions |
| Cell Lines | - |
| Library Construction (kit name) | Ion AmpliSeq Library Kit Plus |
| Fragmentation Methods | - |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | about 120 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000303 |
| Total Data Volume | 29 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Takeshi Sawada
Affiliation: Division of Translational and Clinical Oncology, Cancer Research Institute, Kanazawa University
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (C) | Molecular analysis of traditional serrated adenoma as a candidate precursor of alternative serrated pathway | 16K09304 |
| KAKENHI Grant-in-Aid for Scientific Research on Priority Areas (Research in a proposed research area) | Platform for Advanced Genome Science | 16H06279 (PAGS) |
| Grant-in-Aid from the Takeda Science Foundation | Identification of carcinogenic pathway from serrated precursor lesions to poor-prognosis colon cancer | |
| Grant-in-Aid from the Japanese Foundation for Research and Promotion of Endoscopy | Identification of carcinogenic mechanism of colorectal traditional serrated adenoma and application for endoscopic treatment | |
| Collaborative Research Grant of Cancer Research Institute, Kanazawa University | Molecular analysis of alternative serrated pathway originating from colorectal traditional serrated adenoma |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Significance of gene mutations in the Wnt signaling pathway in traditional serrated adenomas of the colon and rectum | doi: 10.1371/journal.pone.0229262 | JGAD000303 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
