NBDC Research ID: hum0216.v2
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SUMMARY
Aims: To investigate tumor mutation burden for clarification of anti-tumor immuno-response.
Methods: whole exome sequencing analysis
Participants/Materials:
17 patients with lung adenocarcinomas who underwent surgery from 2015-2016
40 patients with malignancies who received PD-1 blackade therapy from 2017 to 2019
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000216 | NGS (Exome) | Controlled-access (Type I) | 2020/02/07 |
| JGAS000244 | NGS (Exome) | Controlled-access (Type I) | 2020/09/25 |
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MOLECULAR DATA
| Participants/Materials |
lung adenocarcinoma (ICD10: C349): 17 cases (matched tumor and non-tumor tissues) |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2500] |
| Library Source | DNAs extracted from tumor or non-tumor tissues from lung adenocarcinomas |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect XT Human All Exon system |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp x 2 |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000302 |
| Total Data Volume | 230 GB (fastq) |
| Comments (Policies) | NBDC policy |
| Participants/Materials |
gastric cancer (ICD10: C16): 26 cases lung cancer (ICD10: C34): 9 cases malignant melanoma (ICD10: C43): 5 cases (matched tumor and non-tumor tissues) |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2500] |
| Library Source | DNAs extracted from tumor or non-tumor tissues from gastric cancers, lung cancers and malignant melanomas |
| Cell Lines | - |
| Library Construction (kit name) | NEBNext Ultra DNA Library Prep Kit |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 120-150 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000344 |
| Total Data Volume | 782 GB (fastq) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Hiroyoshi Nishikawa
Affiliation: Division of Cancer Immunology, Research Institute/Exploratory Oncology Research & Clinical Trial Center (EPOC), National Cancer Center
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| The National Cancer Center Research and Development Fund | Establishment of an advanced system of immune monitoring | 28-A-7 |
| The National Cancer Center Research and Development Fund | Development of novel treatment for immunotherapy-resistant cancer | 31-A-7 |
| Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED) | Establishment of predictive methods for cancer immunotherapy based on the evaluation of cancer cells and immune responses | JP16cm0106301 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Blockade of EGFR improves responsiveness to PD1 blockade in EGFR-mutated non-small cell lung cancer | doi: 10.1126/sciimmunol.aav3937 | JGAD000302 |
| 2 | The PD-1 expression balance between effector and regulatory T cells predicts the clinical efficacy of PD-1 blockade therapies | doi: 10.1038/s41590-020-0769-3 | JGAD000344 |
USRES (Controlled-Access Data)
| Principal Investigator: | Affiliation: | Data in Use (Data Set ID) | Period of Data Use |
|---|---|---|---|
