NBDC Research ID: hum0208.v1
SUMMARY
Aims: To clinically identify recurrence and metastasis prediction markers and establish new molecular target therapies, we conducted a conprehensive genetic analysis of head and neck malignant tumor tissues and patient-derived blood.
Methods: DNA were extracted from tumor tissues and matched normal samples (peripheral blood mononuclear cells: PBMC), and subjected to whole exome sequencing analysis.
Participants/Materials: Bilateral / unilateral cancer of the external auditory canal
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000214 | NGS (Exome) | Controlled-access (Type I) | 2020/01/20 |
* Data users need to apply an application for Using NBDC Human Data to reach the Controlled-access Data.
MOLECULAR DATA
| Participants/Materials |
- bilateral cancer of the external auditory canal (ICD10: C442): 1 case (right and left primary tumor tissues: 2 samples, recurrent tumor tissue: 1 sample) - unilateral cancer of the external auditory canal (ICD10: C442): 9 cases (primary tumor tissues: 9 samples) - matched PBMC: 10 samples |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq X Ten] |
| Library Source | DNAs extracted from tumor and PBMC from patients with cancer of the external auditory canal |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon V6 Kit |
| Fragmentation Methods | included in the above library construction kit |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000300 |
| Total Data Volume | 128 GB (bam [ref: hg19]) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Takashi Nakagawa
Affiliation: Department of Otorhinolaryngology, Kyushu University
Project / Group Name: Comprehensive genetic analysis and database construction for head and neck cancer
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (B) | Development of novel synthesizing treatment strategy by exhaustive research on temporal bone squamous cell carcinoma | 18H02951 |
| KAKENHI Grant-in-Aid for Young Scientists | Elucidation of inflammatory carcinogenesis by comprehensive analysis of inflammasome-related molecules of temporal bone cancer | 18K16895 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Squamous cell carcinoma of the external auditory canal and middle ear: an operation combined with preoperative chemoradiotherapy and a free surgical margin. | doi: 10.1097/01.mao.0000190463.88873.3d | |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
