NBDC Research ID: hum0181.v1
SUMMARY
Aims: Immunohistochemistry of human aldosterone synthase (CYP11B2) has revealed that most of aldosterone is autonomously produced in aldosterone-producing cell clusters (APCCs) beneath the capsule of adult adrenals rather than physiologically in the zona glomerulosa (ZG)1. APCCs have been occasionally found to harbor a somatic mutation of ion channel/pump genes, and number and size of APCCs increase with age until 50 years old2, were reported so far. In this study, we aimed to study if APCCs are increased in individuals older than 50 years where the reninangiotensin system (RAS) is generally suppressed due to aging.
1: J Clin Endocrinol Metab 2010;95,2296-305, 2: Int J Endocrinol 2016;7834356
Methods: Archival adrenal gland were immunohistochemically analyzed using antibodies for aldosterone-synthase (CYP11B2) and 3β-hydroxysteroid dehydrogenase (3β-HSD). APCC areas per whole adrenal area (AA / WAA) and number of APCC per whole adrenal area (NOA / WAA) were measured and used for statistical analyses.
Participants/Materials: 85 anatomical individuals in the Saitama Medical University Hospital, who died at ages from 50 to 103 years.
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| hum0181.v1.apcc.v1 | histological data (Immunostaining for CYP11B2, 3β-HSD, hematoxylin and eosin staining) | Unrestricted-access | 2019/09/25 |
*When the research results including the data which were downloaded from NHA/DRA, are published or presented somewhere, the data user must refer the papers which are related to the data, or include in the acknowledgment. Learn more
MOLECULAR DATA
| Participants/Materials | Anatomical cases who were more than 50 years old (50 to 103 years): 85 cases |
| Targets | - |
| Organ/Tissue | adrenal glands |
| Immobilization method | 10% formalin |
| Antigen activation method (temperature, reagent, PH, time) | EnVision FLEX Kit, High pH (Agilent, ＃K8002) |
| Blocking method (reagent, time) | EnVision FLEX Kit, High pH (Agilent, ＃K8002) |
| Primary antibodies for IHC（manufacturer, Cat. #, conc., temp., time） |
CYP11B2：CYPIIB2-4Ell-1G7 (in house antibody) HSD3B2：see ref2 |
| Secondary antibodies for IHC（manufacturer, Cat. #, conc., temp., time） |
CYP11B2：EnVision FLEX Kit, High pH (Agilent, ＃K8002) HSD3B2：EnVision FLEX Kit, High pH (Agilent, ＃K8002) |
| Chromogen (reagent, time) | EnVision FLEX Kit, High pH (Agilent, ＃K8002) |
| Washing (reagent, time) | EnVision FLEX Kit, High pH (Agilent, ＃K8002) |
| NBDC Dataset ID |
hum0181.v1.apcc.v1 - Download files per staining |
| Total Data Volume | 147 GB (svs) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Koshiro Nishimoto
Affiliation: Saitama Medical University International Medical Center, Department of UroOncology
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (C) | - | 15K10650 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | - | 18K09205 |
| KAKENHI Grant-in-Aid for Scientific Research (C) | - | 17K09890 |
| Yamaguchi Endocrine Research Foundation Grant | - | - |
| Okinaka Memorial Institute for Medical Research Grant | - | - |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Expression of aldosterone synthase cyp11b2 was inversely correlated with longevity. | doi: 10.1016/j.jsbmb.2019.04.010 | - |
| 2 | Development of monoclonal antibodies against the human 3beta-hydroxysteroid dehydrogenase/isomerase isozymes. | doi: 10.1016/j.steroids.2017.08.011 | - |
