NBDC Research ID: hum0139.v1
SUMMARY
Aims: To address how status of KRAS in iPS cells impacts upon self-renewal and differentiation propensity
Methods: Roles of KRAS on stemness were investigated in the context of induced pluripotent stem cells (iPSCs). KRAS mutant (G13C/WT) and wild-type isogenic (WT/WT) iPSCs from a Ras-associated autoimmune leukoproliferative disorder (RALD) patient were used. Retention of self-renewal and capacity for neuronal differentiation were compared.
Participants/Materials: A RALD patient-derived iPSC clones (one with KRAS G13C mutation and the other one with no mutation derived from the same patient).
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000136 | NGS (Exome) | Controlled-access (Type I) | 2018/07/10 |
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MOLECULAR DATA
| Participants/Materials |
iPSCs from 1 RALD patient (ICD10: D763) KRAS mutant (G13C/WT) iPSCs: 1 sample wild-type isogenic (WT/WT) iPSCs: 1 sample |
| Targets | Exome |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 1500] |
| Library Source | DNAs extracted from each iPSC |
| Cell Lines | - |
| Library Construction (kit name) | SureSelect Human All Exon V5 |
| Fragmentation Methods | SureSelect QXT Human All Exon V5: Enzymatic fragmentation (transposase-based library prep) |
| Spot Type | Paired-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 100 bp x 2 |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000205 |
| Total Data Volume |
32 GB Data files: 4 (fastq) Analysis files: 2 (bam [ref: hg19]) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Makoto Otsu
Affiliation: Institute of Medical Science, University of Tokyo
Project / Group Name: The program for intractable diseases research utilizing disease-specific iPS cells
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Research Center Network for Realization of Regenerative Medicine, Japan Agency for Medical Research and Development (AMED) | The program for intractable diseases research utilizing disease-specific iPS cells | JP16bm0609006 JP17bm0804004 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Status of KRAS in iPS cells impacts upon self-renewal and differentiation propensity. | doi: 10.1016/j.stemcr.2018.06.008 | JGAD000205 |
| 2 |
USERS (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
