NBDC Research ID: hum0129.v1
SUMMARY
Aims: Functional analysis of neural cells derived from CHARGE syndrome patients' fibroblast cells
Methods: Establishment of iPS cells from CHARGE syndrome patients' fibroblast cells
Participants/Materials: iPS cells or neuroepithelial-like stem cells induced from iPS cells established from CHARGE syndrome patients' skin fibroblast cells
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000131 | NGS (ChIP-seq) | Controlled-access (Type I) | 2018/04/06 |
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MOLECULAR DATA
| Participants/Materials |
CHARGE syndrome patient (ICD10: Q878): 3 cases (2 samples each, total 4 samples) |
| Targets | ChIP-seq |
| Target Loci for Capture Methods | - |
| Platform | Illumina [HiSeq 2500] |
| Library Source |
DNAs extracted from long-term self-renewing neuroepithelial-like stem cells induced from iPS cells established from skin fibroblasts of CHARGE syndrome patients anti-histone antibody (H3K27ac) was used for immunoprecipitation |
| Cell Lines | - |
| Library Construction (kit name) | KAPA Hyper Preparation kit |
| Fragmentation Methods | Ultrasonic fragmentation (Covaris S2) |
| Spot Type | Single-end |
| Read Length (without Barcodes, Adaptors, Primers, and Linkers) | 50 bp |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000142 |
| Total Data Volume |
9 GB Data files : 16 (fastq) Analysis files : 8 (wig [ref: hg19]) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Hideyuki Okano
Affiliation: Keio University School of Medicine
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| Research Center Network for Realization of Regenerative Medicine Centers for Clinical Application Research on Specific Disease/Organ, Japan Agency for Medical Research and Development (AMED) | Regenerative medicine for spinal cord injury and stroke using human induced pluripotent stem cell-derived neural precursor cells | JP16bm0204001 |
| Research Project for Practical Application of Regenerative Medicine, Japan Agency for Medical Research and Development (AMED) | Mechanism of tumorigenesis of human iPSC-derived neural progenitor/stem cells | JP15bk0104009 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Chromatin remodeler CHD7 regulates the stem cell identity of human neural progenitors | doi: 10.1101/gad.301887.117 | JGAD000142 |
| 2 |
USRES (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
