NBDC Research ID: hum0117.v1
SUMMARY
Aims: Detecting Neuron-specifc methylation changes in Alzheimer's disease brain
Methods: Illumina Infinium® Human Methylation 450K BeadChip
Participants/Materials: 30 Alzheimer's diseasepatients and 30 healthy controls
| Dataset ID | Type of Data | Criteria | Release Date |
|---|---|---|---|
| JGAS000125 | Methylation array | Controlled-access (Type I) | 2017/12/11 |
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MOLECULAR DATA
| Participants/Materials |
Alzheimer's disease (ICD10：G309): 30 cases Healthy control: 30 individuals |
| Targets | Methylation array |
| Target Loci for Capture Methods | - |
| Platform | Illumina Infinium® Human Methylation 450K BeadChip |
| Library Source | DNAs extracted from autopsied brain tissue |
| Cell Lines | - |
| Library Construction (kit name) |
Bisulfite Conversion: EZ DNA Methylation™ Kit (Zymo Research) Methylation analysis: Infinium® Human Methylation 450K BeadChip (Illumina) |
| Algorithms for Calculating Methylation-rate (software) | iScan scanner (Illumina) |
| Filtering Methods | - |
| Normalization of microarray | - |
| Probe Number | 487,557 probes (raw data) |
| Japanese Genotype-phenotype Archive Dataset ID | JGAD000136 |
| Total Data Volume | 1 TB (idat) |
| Comments (Policies) | NBDC policy |
DATA PROVIDER
Principal Investigator: Atsushi Iwata
Affiliation: The University of Tokyo Hospital
Project / Group Name: -
Funds / Grants (Research Project Number):
| Name | Title | Project Number |
|---|---|---|
| KAKENHI Grant-in-Aid for Scientific Research (B) | A novel neuro-epigenetic approach to the pathogenic mechanism of the neurodegenerative dementia diseases | 16H05319 |
| KAKENHI Grant-in-Aid for Young Scientists (B) | Analysis of DNA damage mechanism in the novel cellular and mouse model of Alzheimer’s disease | 17K16113 |
| Strategic Research Program for Brain Sciences, Japan Agency for Medical Research and Development (AMED) | Development of a new disease-modifying therapy and early diagnostic method of Alzheimer’s disease based on a cause of amyloid deposition observed in sporadic type | JP16dm0107069 |
PUBLICATIONS
| Title | DOI | Dataset ID | |
|---|---|---|---|
| 1 | Neuron-specific methylome analysis reveals epigenetic regulation and tau-related dysfunction of BRCA1 in Alzheimer's disease. | doi: 10.1073/pnas.1707151114 | JGAD000136 |
| 2 | - | - | - |
USERS (Controlled-access Data)
| Principal Investigator | Affiliation | Country/Region | Research Title | Data in Use (Dataset ID) | Period of Data Use |
|---|---|---|---|---|---|
