hum0112 Release Note

hum0112.v1

NGS (PBAT-seq)

DNAs extracted from trophoblast stem cell were used for PBAT-seq analyses (fastq format files). Libraries were prepared by Post-Bisulfite Adaptor-Tagging (PBAT) and sequencing was performed with Illumina [HiSeq 1500] (101 bp, single-end).

NGS (ChIP-seq)

DNAs extracted from trophoblast stem cell were used for ChIP-seq analyses (fastq format files). Anti H3K4me3, H3K4me1, H3K9me3, H3K27ac, H3K27me3, H3K36me3 antibodies were used for immunoprecipitation. ChIP-Seq libraries were prepared by using Ovation Ultralow System V2 kit (NuGEN) and sequenced using Illumina [HiSeq 2500] (126 bp, paired-end).

NGS (RNA-seq)

mRNAs extracted from trophoblast stem cell were used for RNA-seq analyses (fastq format files). Libraries were prepared by using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequencing was performed with Illumina [HiSeq 2500] (101 bp, paired-end).

NGS (small RNA-seq)

RNAs extracted from trophoblast stem cell were used for miRNA-seq analyses (fastq format files). Libraries were prepared by using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) and sequencing was performed with Illumina [HiSeq 1500] (64 bp, single-end).

Note: The processed data will soon be released on the IHEC Data Portal (http://ihec-epigenomes.org/)